Difference between revisions of "Part:BBa K2924009"

 
 
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<partinfo>BBa_K2924009 short</partinfo>
 
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Ribosome binding site* (RBS*)
  
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===Usage and Biology===
 
===Usage and Biology===
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The RBS is the location in which a ribosome binds to the mRNA and initiates the translation. The effectiveness of the translation depends amongst other things <sup>1</sup> on the base-pairing potential between the Shine-Dalgarno sequence and the anti-Shine-Dalgarno sequence at the 3’ end of the 16S rRNA sequence and it depends on the space between the A of the core Shine-Dalgarno sequence and the first base of the start codon of the gene of interest <sup>1, 2</sup>.
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RBS* was created by Heidorn (2011), is complementary to the anti-Shine-Dalgarno of <i>Synechocystis sp.</i> PCC 6803 and contains an optimal space of 9 base pairs <sup>2</sup>. It has been shown that RBS* is specifically in <i>Synechocystis sp.</i> PCC 6803 very strong <sup>2</sup>. This was tested with <i>Escherichia coli</i> and <i>Synechocystis sp.</i> PCC 6803 each containing standard biological parts <html><a href="https://parts.igem.org/Part:BBa_B0030">BBa_B0030</a></html>, <html><a href="https://parts.igem.org/Part:BBa_B0032">BBa_B0032</a></html>, <html><a href="https://parts.igem.org/Part:BBa_B0034">BBa_B0034</a></html> and RBS*. Due to fluorescence of GFPmut3B, the transitional effectiveness could be measured and compared <sup>2</sup>.
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The RBS* can therefore be used in <i>Synechocystis</i> for our approach to highly efficient translate target thioesterases from heterologous organism in the constructs <html><a href="https://parts.igem.org/Part:BBa_K2924010">BBa_K2924010</a></html>, <html><a href="https://parts.igem.org/Part:BBa_K2924011">BBa_K2924011</a></html>, <html><a href="https://parts.igem.org/Part:BBa_K2924012">BBa_K2924012</a></html>, and <html><a href="https://parts.igem.org/Part:BBa_K2924013">BBa_K2924013</a></html>.
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<partinfo>BBa_K2924009 parameters</partinfo>
 
<partinfo>BBa_K2924009 parameters</partinfo>
 
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===References===
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[1]: Ringquist, S., Shinedlind, S., Barrick, D., Green, L., Binkley, J., Stormo, G. D., Gold, L. "Translation initiation in Escherichia coli: sequences within the ribosome‐binding site." <i>Molecular microbiology</i> 6.9 (1992): 1219-1229.
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[2]:  Heidorn T., Camsund, D., Huang, H.-H., Lindberg, P., Oliveira, P., Stensjö, K., Lindberg, P. "Synthetic biology in cyanobacteria: engineering and analyzing novel functions." <i>Methods in enzymology.</i> Vol. 497. Academic Press, 2011. 539-579.

Latest revision as of 19:48, 20 October 2019


RBS*

Ribosome binding site* (RBS*)

Usage and Biology

The RBS is the location in which a ribosome binds to the mRNA and initiates the translation. The effectiveness of the translation depends amongst other things 1 on the base-pairing potential between the Shine-Dalgarno sequence and the anti-Shine-Dalgarno sequence at the 3’ end of the 16S rRNA sequence and it depends on the space between the A of the core Shine-Dalgarno sequence and the first base of the start codon of the gene of interest 1, 2.

RBS* was created by Heidorn (2011), is complementary to the anti-Shine-Dalgarno of Synechocystis sp. PCC 6803 and contains an optimal space of 9 base pairs 2. It has been shown that RBS* is specifically in Synechocystis sp. PCC 6803 very strong 2. This was tested with Escherichia coli and Synechocystis sp. PCC 6803 each containing standard biological parts BBa_B0030, BBa_B0032, BBa_B0034 and RBS*. Due to fluorescence of GFPmut3B, the transitional effectiveness could be measured and compared 2.

The RBS* can therefore be used in Synechocystis for our approach to highly efficient translate target thioesterases from heterologous organism in the constructs BBa_K2924010, BBa_K2924011, BBa_K2924012, and BBa_K2924013.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1]: Ringquist, S., Shinedlind, S., Barrick, D., Green, L., Binkley, J., Stormo, G. D., Gold, L. "Translation initiation in Escherichia coli: sequences within the ribosome‐binding site." Molecular microbiology 6.9 (1992): 1219-1229.

[2]: Heidorn T., Camsund, D., Huang, H.-H., Lindberg, P., Oliveira, P., Stensjö, K., Lindberg, P. "Synthetic biology in cyanobacteria: engineering and analyzing novel functions." Methods in enzymology. Vol. 497. Academic Press, 2011. 539-579.