Difference between revisions of "Part:BBa K864404"

 
 
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<partinfo>BBa_K864404 short</partinfo>
 
<partinfo>BBa_K864404 short</partinfo>
  
cjBlue is a blue (&#955;[maxabs]=610nm) chromoprotein from the sea anemone Cnidopus japoincus, as reported by (Chan et. al., 2006). We synthesized cjBlue codon optimized for expression in e.coli and observed a turquoise colour, wavelength approximately 5*10^2nm. This part is also submitted as a functionally active variant with J23110 and B0034
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This is an expandation of chromoprotein cjBlue [https://parts.igem.org/wiki/index.php/Part:BBa_K592011 BBa_K592011]. It provides a turqoise color visible to the naked eye. The sequence is codon optimized for expression in E coli.
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It has previously been assembled with another promotor ([https://parts.igem.org/Part:BBa_K592022 BBa_K592022]).
  
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===Usage and Biology===
 
===Usage and Biology===
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This part is useful as a reporter.
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[[Image:CjBlue_plate_small.jpg|420px]]
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[[Image:UUChromo.jpg|500px]]
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'''iGEM12_Uppsala_University:'''<br>
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''Left picture:'' Visual color expression of cjBlue with a different promotor([https://parts.igem.org/Part:BBa_K592022 BBa_K592022]). <br>
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''Right picture:'' Pellet of chromoproteins eforRed <partinfo>BBa_K592012</partinfo>, RFP <partinfo>BBa_E1010</partinfo>, cjBlue <partinfo>BBa_K592011</partinfo>, aeBlue <partinfo>BBa_K864401</partinfo>, amilGFP <partinfo>BBa_K592010</partinfo> and amilCP <partinfo>BBa_K592009</partinfo>.
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K864404 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K864404 SequenceAndFeatures</partinfo>
  
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===Newcastle 2019 Characterisation===
  
 
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Latest revision as of 17:19, 20 October 2019

cjBlue Coding Device

This is an expandation of chromoprotein cjBlue BBa_K592011. It provides a turqoise color visible to the naked eye. The sequence is codon optimized for expression in E coli.

It has previously been assembled with another promotor (BBa_K592022).

Usage and Biology

This part is useful as a reporter.

CjBlue plate small.jpg UUChromo.jpg

iGEM12_Uppsala_University:
Left picture: Visual color expression of cjBlue with a different promotor(BBa_K592022).
Right picture: Pellet of chromoproteins eforRed BBa_K592012, RFP BBa_E1010, cjBlue BBa_K592011, aeBlue BBa_K864401, amilGFP BBa_K592010 and amilCP BBa_K592009.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Newcastle 2019 Characterisation