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===Usage and Biology===
  
 
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<div class="figurtext"style=font-size:80%;><i><b>Figure 1.</b> The picture to the left depict a <i>E. coli</i> BL21 (DE3) culture expressing pCons-eforRed (left tube) versus a negative control (right tube) with <i>E. coli</i> BL21 (DE3) containing no construct after 48 hours incubation in 37°C. The top right picture displays centrifuged cultures of <i>E. coli</i> BL21 (DE3) with pCons-eforRed in UV-light. The right picture is a culture of <I>E. coli</I> BL21 (DE3) expressing eforRed which has been incubated for 48 hours in 37 degrees Celsius and centrifuged at 12 000g for 10 min. The result is a pellet of eforRed expressing bacteria with a Burgundy color (bottom right). The same pellet in the top right was put on a UV table (302 nm) which resulted in a pink glowing pellet (top right).</i></div>
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<div class="figurtext" style=font-size:90%;><b>Figure 1.</b> The picture to the left depicts a cell culture of BL21 (DE3) pCons-eforRed (left tube) versus a negative control (right tube) with BL21 (DE3). The top right picture displays a pellet of BL21 (DE3) with pCons-eforRed in UV-light. The right picture is a pellet of BL21 (DE3) in white light.</div>
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Revision as of 15:30, 20 October 2019

J23110-B0034-eforRed

eforRed eforRed is previously described as BBa_K592012. We submitted a functionally active variant with J23110 and B0034.


Eforred plate small.jpg UUChromo.jpg


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Note: This part did not have a reference sequence. A reference sequence has since been added based on the part's documentation; a composite part using the following basic parts: No part name specified with partinfo tag. - BBa_B0034 - BBa_K592012- iGEM HQ