Difference between revisions of "Part:BBa K3187045"

 
 
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<partinfo>BBa_K3187045 short</partinfo>
 
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This part is a generator for the P22 bacteriophage capsid containing both capsomers coat protein and scaffold protein under the control of a TetR/tetA promoter. Expression can be induced by addition of anhydrotetracyclin (AHT). This part was used together with <a
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                    href=" https://parts.igem.org/Part:BBa_K3187044" target="_blank">BBa_K3187044</a> for the dual expression of the P22 capsid with different ratios of LPETGG-tagged coat protein to untagged coat protein. The potential of fine tuning the expression levels of both parts was tested via <a
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                    href=" https://parts.igem.org/Part:BBa_K3187011" target="_blank">BBa_K3187011</a>.
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<span class='h3bb'>Sequence and Features</span>
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<h2><span class='h3bb'>Sequence and Features</span></h2>
 
<partinfo>BBa_K3187045 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3187045 SequenceAndFeatures</partinfo>
  

Latest revision as of 08:56, 20 October 2019


P22 Bacteriophage Coat and Scaffolding Protein (tetR/tetA promotor)

This part is a generator for the P22 bacteriophage capsid containing both capsomers coat protein and scaffold protein under the control of a TetR/tetA promoter. Expression can be induced by addition of anhydrotetracyclin (AHT). This part was used together with BBa_K3187044 for the dual expression of the P22 capsid with different ratios of LPETGG-tagged coat protein to untagged coat protein. The potential of fine tuning the expression levels of both parts was tested via BBa_K3187011.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 76
    Illegal EcoRI site found at 1395
    Illegal PstI site found at 1931
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 76
    Illegal EcoRI site found at 1395
    Illegal NheI site found at 1936
    Illegal PstI site found at 1931
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 76
    Illegal EcoRI site found at 1395
    Illegal BamHI site found at 1389
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 76
    Illegal EcoRI site found at 1395
    Illegal PstI site found at 1931
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 76
    Illegal EcoRI site found at 1395
    Illegal PstI site found at 1931
    Illegal NgoMIV site found at 1783
  • 1000
    COMPATIBLE WITH RFC[1000]