Difference between revisions of "Part:BBa K3187043"
 
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This part is a generator for the P22 bacteriophage scaffold protein fused to a super folder GFP. This part was used together with <a
 
This part is a generator for the P22 bacteriophage scaffold protein fused to a super folder GFP. This part was used together with <a
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                     href=" https://parts.igem.org/Part:BBa_K3187044" target="_blank">BBa_K3187044</a> for the dual expression of the P22 scaffold protein and the P22 coat protein fused to a LPETGG-tag for Sortase-mediated ligation of GGGG-tagged proteins to the surface of P22 Virus-like particles.
+
                     href=" https://parts.igem.org/Part:BBa_K3187044" target="_blank">BBa_K3187044</a> for the dual expression of the P22 scaffold protein and the P22 coat protein fused to a LPETGG-tag for sortase-mediated ligation of GGGG-tagged proteins to the surface of P22 Virus-like particles. The potential of fine tuning the expression levels of both parts was tested via <a
 +
                    href=" https://parts.igem.org/Part:BBa_K3187011" target="_blank">BBa_K3187011</a>.
 
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<span class='h3bb'>Sequence and Features</span>
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<h2><span class='h3bb'>Sequence and Features</span></h2>
 
<partinfo>BBa_K3187043 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3187043 SequenceAndFeatures</partinfo>
  

Latest revision as of 08:53, 20 October 2019


Superfolder GFP + P22 Bacteriophage Scaffolding Protein Generator (tetR/tetA promotor)

This part is a generator for the P22 bacteriophage scaffold protein fused to a super folder GFP. This part was used together with BBa_K3187044 for the dual expression of the P22 scaffold protein and the P22 coat protein fused to a LPETGG-tag for sortase-mediated ligation of GGGG-tagged proteins to the surface of P22 Virus-like particles. The potential of fine tuning the expression levels of both parts was tested via BBa_K3187011.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 76
    Illegal PstI site found at 1334
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 76
    Illegal NheI site found at 1339
    Illegal PstI site found at 1334
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 76
    Illegal BamHI site found at 796
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 76
    Illegal PstI site found at 1334
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 76
    Illegal PstI site found at 1334
    Illegal NgoMIV site found at 1185
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 94