Difference between revisions of "Part:BBa K3114025"
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Following the coding region is iGEM Calgary's universal spacer sequence and 6XHis tag site[https://parts.igem.org/Part:BBa_K3114014 (BBa_K3114014)].The universal spacer was utilized in an attempt to mitigate any adverse effects that the his tag may have on the protein's function, as hypothesized in Meguro et al. 2011. | Following the coding region is iGEM Calgary's universal spacer sequence and 6XHis tag site[https://parts.igem.org/Part:BBa_K3114014 (BBa_K3114014)].The universal spacer was utilized in an attempt to mitigate any adverse effects that the his tag may have on the protein's function, as hypothesized in Meguro et al. 2011. | ||
| − | ===Sequences and Features | + | ===Sequences and Features=== |
<partinfo>BBa_K3114025 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3114025 SequenceAndFeatures</partinfo> | ||
Revision as of 02:23, 20 October 2019
7-Hydroxymethyl Chlorophyll A Reductase (7-HCAR) inducible circuit
Usage and Biology
7-hydroxymethyl chlorophyll a reductase (7-HCAR) is an enzyme which catalyzes the reaction converting 7-hydroxymethyl chlorophyll a into chlorophyll a. (Meguro et al. 2011)
BBa_K3114025 is a part consisting of multiple components of iGEM Calgary's 2019 Biobrick submission kit, as well as a few other well-characterized parts. The coding region contains the gene encoding for 7-HCAR[1].A T7 promoter(BBa_I719005)was used in conjunction with a strong ribosome binding site(BBa_B0030). Following the coding region is iGEM Calgary's universal spacer sequence and 6XHis tag site(BBa_K3114014).The universal spacer was utilized in an attempt to mitigate any adverse effects that the his tag may have on the protein's function, as hypothesized in Meguro et al. 2011.
Sequences and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1651
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 915
Illegal AgeI site found at 339
Illegal AgeI site found at 432 - 1000COMPATIBLE WITH RFC[1000]
References
Meguro, M., Ito, H., Takabayashi, A., Tanaka, R., & Tanaka, A. (2011). Identification of the 7-hydroxymethyl chlorophyll a reductase of the chlorophyll cycle in Arabidopsis. The Plant Cell, 23(9), 3442-3453.