Difference between revisions of "Part:BBa K3015004"

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The iGEM Team BOKU-Vienna improved the Part [[Part:BBa_K784005|BBa_K784005]] by designing a Theophylline Riboswitch that works in a transcriptional way, instead of a translational way.
 
The iGEM Team BOKU-Vienna improved the Part [[Part:BBa_K784005|BBa_K784005]] by designing a Theophylline Riboswitch that works in a transcriptional way, instead of a translational way.
 
The coding sequence does not have to be scarless fused to the Riboswitch, which makes cloning easier.
 
The coding sequence does not have to be scarless fused to the Riboswitch, which makes cloning easier.
 
+
To test the probabilities of the Riboswitch we conducted measurements by fusing the Ribswitch upstream to a Promotor and downstream to an RBS and GFP. You can find those measurements on the Regsitry-Page of the described Composite Part [[Part:BBa_K3015007|BBa_K3015007]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 11:55, 19 October 2019


Theophylline_Riboswitch (transcriptional)

The iGEM Team BOKU-Vienna improved the Part BBa_K784005 by designing a Theophylline Riboswitch that works in a transcriptional way, instead of a translational way. The coding sequence does not have to be scarless fused to the Riboswitch, which makes cloning easier. To test the probabilities of the Riboswitch we conducted measurements by fusing the Ribswitch upstream to a Promotor and downstream to an RBS and GFP. You can find those measurements on the Regsitry-Page of the described Composite Part BBa_K3015007

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]