Difference between revisions of "Part:BBa K2961000:Design"

 
 
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===Design Notes===
 
===Design Notes===
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One must ensure that the target sequence does not contain the Cas12a PAM sequence, but rather that in the human genome, the PAM sequence of Cas12a is just upstream of it. (The PAM sequence for Cas12a is TTTN)
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===Source===
 
===Source===
  
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The fixed loop part was adapted from the NEB website, as per their instructions (https://international.neb.com/faqs/2018/05/03/how-do-i-design-a-guide-rna-for-use-with-engen-lba-cas12a).
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This CFTR gene segment was generated through CHOPCHOP by Harvard, and verified through BLAST and other genome browsing softwares. Note that this means there is a convenient PAM site near the gene on the genome that can be used by Cpf1 for targeting using gRNA.
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The segment that is specific to the CFTR gene variant was generated through CHOPCHOP by Harvard, and verified through BLAST and other genome browsing softwares.
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===References===
 
===References===

Latest revision as of 01:40, 17 October 2019


CFTR Mutant Gene Segment (rs75389940_G)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

This CFTR gene segment was generated through CHOPCHOP by Harvard, and verified through BLAST and other genome browsing softwares. Note that this means there is a convenient PAM site near the gene on the genome that can be used by Cpf1 for targeting using gRNA.

References