Difference between revisions of "Part:BBa K2961001"

 
 
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<partinfo>BBa_K2961001 short</partinfo>
 
<partinfo>BBa_K2961001 short</partinfo>
  
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This part encodes for a guide RNA that can be used with Cpf1. The sequence includes a crRNA repeat, known as the fixed loop, to allow Lab Cas12a to recognize the gRNA (different manufacturers may produce Cas12a that are optimized for recognizing different fixed loops; the sequence posted here is optimized for NEB Cas12a). The sequence also includes a segment that is specific to the wild type  CFTR gene.
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This part encodes for a segment of the genome corresponding to the wildtype CFTR gene.
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This part is contrasted by BBa_K2961000, which encodes for the same segment but with a single nucleotide mutation that results in a variant of cystic fibrosis.
  
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This part may be used to simply manufacture this gRNA. It can be used in conjunction with template DNA extracted from patients or synthesized in a lab to allow Cas12a to recognize and bind to the wild type CFTR gene. This may then be paired with further downstream effects (such as Cas12a activity causing fluorescence of some kind) to detect DNA samples that do not have the cystic fibrosis disease.
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Note that this part of the CFTR gene is conveniently located near a PAM site for Cpf1, so can be used in gRNA design.
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This part is also useful as a control for use in conjunction with BBa_K2961000
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Latest revision as of 01:37, 17 October 2019


CFTR Wildtype gene segment

This part encodes for a segment of the genome corresponding to the wildtype CFTR gene. This part is contrasted by BBa_K2961000, which encodes for the same segment but with a single nucleotide mutation that results in a variant of cystic fibrosis.

Note that this part of the CFTR gene is conveniently located near a PAM site for Cpf1, so can be used in gRNA design.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]