Difference between revisions of "Part:BBa K2961001:Design"

 
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===Design Notes===
 
===Design Notes===
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One must ensure that the target sequence does not contain the Cas12a PAM sequence, but rather that in the human genome, the PAM sequence of Cas12a is just upstream of it. (The PAM sequence for Cas12a is TTTN)
 
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===Source===
 
===Source===

Revision as of 01:34, 17 October 2019


CFTR Wildtype gene segment


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Unknown
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Unknown


Design Notes

Source

he fixed loop part was adapted from the NEB website, as per their instructions (https://international.neb.com/faqs/2018/05/03/how-do-i-design-a-guide-rna-for-use-with-engen-lba-cas12a). The segment that is specific to the CFTR gene wild type variant was generated through CHOPCHOP by Harvard, and verified through BLAST and other genome browsing softwares.

References