Difference between revisions of "Part:BBa K2922036"
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<partinfo>BBa_K2922036 short</partinfo>
 
<partinfo>BBa_K2922036 short</partinfo>
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===Summary===
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This is a composite part consist of a T7 promoter (<partinfo>BBa_K525998</partinfo>), CDS of colicin-E1 (<partinfo>BBa_K2922024</partinfo>), CDS of colicin-E1 immunity protein (<partinfo>BBa_K2922025</partinfo>) and CDS of lysis protein (<partinfo>BBa_K2922026</partinfo>). Each CDS has an RBS (<partinfo>BBa_B0034</partinfo>) behind. T7 promoter could be induced by IPTG or lactose in Escherichia coli BL21 strain and then express all proteins mentioned. E coli that can`t express colicin-E1 immunity protein would be killed by colicin-E1, colicin-E1 immunity protein is used to protect itself from attack of extracellular colicin-E1 and kil protein helps the release of colicin-E1. This part is constructed in the aim of achieve our “aggressive” design.
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<br>
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===Ientification===
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<br>
  
 
<partinfo>BBa_K2922036 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2922036 SequenceAndFeatures</partinfo>

Revision as of 17:02, 14 October 2019

The colicin-E1 operon under T7 promoter control

Summary

This is a composite part consist of a T7 promoter (BBa_K525998), CDS of colicin-E1 (BBa_K2922024), CDS of colicin-E1 immunity protein (BBa_K2922025) and CDS of lysis protein (BBa_K2922026). Each CDS has an RBS (BBa_B0034) behind. T7 promoter could be induced by IPTG or lactose in Escherichia coli BL21 strain and then express all proteins mentioned. E coli that can`t express colicin-E1 immunity protein would be killed by colicin-E1, colicin-E1 immunity protein is used to protect itself from attack of extracellular colicin-E1 and kil protein helps the release of colicin-E1. This part is constructed in the aim of achieve our “aggressive” design.


Ientification



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 544
    Illegal AgeI site found at 1902
    Illegal AgeI site found at 1959
    Illegal AgeI site found at 2091
  • 1000
    COMPATIBLE WITH RFC[1000]