Difference between revisions of "Part:BBa K3174002"
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<partinfo>BBa_J23104</partinfo> is part of the Anderson Series of constitutive promoters designed by iGEM2006_Berkeley. It was used by Manchester in 2017 to create <partinfo>BBa_K2213008</partinfo>. | <partinfo>BBa_J23104</partinfo> is part of the Anderson Series of constitutive promoters designed by iGEM2006_Berkeley. It was used by Manchester in 2017 to create <partinfo>BBa_K2213008</partinfo>. | ||
| − | <partinfo>BBa_B0034</partinfo> is an RBS based on the Elowitz repressilator and was designed | + | <partinfo>BBa_B0034</partinfo> is an RBS based on the Elowitz repressilator and was designed in 2003. Its relative strength was characterized by IIT Madras in 2016 and by Team Warsaw in 2010. |
Revision as of 20:33, 19 August 2019
Blue Fluorescent Protein with Strong Promoter and Strong RBS
This part has the blue fluorescent protein (mTagBFP) gene inserted downstream of a strong promoter and strong ribosome binding site.
BBa_J23104 is part of the Anderson Series of constitutive promoters designed by iGEM2006_Berkeley. It was used by Manchester in 2017 to create BBa_K2213008.
BBa_B0034 is an RBS based on the Elowitz repressilator and was designed in 2003. Its relative strength was characterized by IIT Madras in 2016 and by Team Warsaw in 2010.
Usage and Biology
UT Austin iGEM 2019 Team transformed this part into the E.coli DH10B burden monitor (https://2019.igem.org/Team:Austin_UTexas
) and used it as a standard with which cellular burden could be quantified (by means of a GFP expression vs growth rate model) for other BioBricks that had been similarly transformed into the burden strain.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]