Difference between revisions of "Part:BBa K2610041"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | We, iGEM Leiden 2018, have combined this naturally occurring constitutive promoter from Escherichia coli with the blue chromoprotein AmilCP (BBa_K592009) in order for us to compare it to our novel stress promoter+chromoprotein constructs | + | We, iGEM Leiden 2018, have combined this naturally occurring constitutive promoter ([[https://parts.igem.org/Part:BBa_K2610040 BBa_K2610040]) from <i> Escherichia coli </i> with the blue chromoprotein AmilCP ([[https://parts.igem.org/Part:BBa_K592009 BBa_K592009]) in order for us to compare it to our novel stress promoter+chromoprotein constructs. |
| − | Moreover, GapA being a natural occurring promoter there are still possibilities for optimization through radiation. This way variations of this promoter can provide access to an even wider spectrum of colors. | + | Contrary to our stress promoters, this promoter has a constitutive high basal expression level. In contrast to many synthetic promoters, this novel promoter provides an indication of high levels of natural expression. Therefore, this promoter can be used as a future positive control of reporter production - such as GFP - in a natural situation. Moreover, GapA being a natural occurring promoter means that there are still possibilities for optimization through radiation. This way variations of this promoter can provide access to an even wider spectrum of colors. |
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| − | <span style="font-size:1em"><b>Figure 1.</b> Constitutively expressed AmilCP </span> | + | [[File:T--Leiden--chromo.png|600px]] |
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| + | <span style="font-size:1em"><b>Figure 1.</b> Constitutively expressed pGapA-AmilCP compared to non-transformed DH5a and pGapA-spis-Pink (pink chromoprotein)</span> | ||
===References=== | ===References=== | ||
Latest revision as of 01:55, 18 October 2018
pGapA-AmilCP
Promoter GapA is naturally occurring constitutive promoter in Escherichia coli.
Usage and Biology
We, iGEM Leiden 2018, have combined this naturally occurring constitutive promoter ([BBa_K2610040) from Escherichia coli with the blue chromoprotein AmilCP ([BBa_K592009) in order for us to compare it to our novel stress promoter+chromoprotein constructs.
Contrary to our stress promoters, this promoter has a constitutive high basal expression level. In contrast to many synthetic promoters, this novel promoter provides an indication of high levels of natural expression. Therefore, this promoter can be used as a future positive control of reporter production - such as GFP - in a natural situation. Moreover, GapA being a natural occurring promoter means that there are still possibilities for optimization through radiation. This way variations of this promoter can provide access to an even wider spectrum of colors.
Figure 1. Constitutively expressed pGapA-AmilCP compared to non-transformed DH5a and pGapA-spis-Pink (pink chromoprotein)
References
Munjal, N., Jawed, K., Wajid, S., & Yazdani, S. S. (2015). A constitutive expression system for cellulase secretion in Escherichia coli and its use in bioethanol production. PloS one, 10(3), e0119917.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 140