Difference between revisions of "Part:BBa K2656106"
 
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Transcriptional unit assembled with a one-pot [http://2018.igem.org/Team:Valencia_UPV/Design Level 1] Golden Gate reaction using BsaI type IIS endonuclease (Figure 1).
 
Transcriptional unit assembled with a one-pot [http://2018.igem.org/Team:Valencia_UPV/Design Level 1] Golden Gate reaction using BsaI type IIS endonuclease (Figure 1).
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[[File:T--Valencia_UPV_TU_UPV2018.png|500px|thumb|none|alt=TU.|Figure 1. Transcriptional unit assembled. Golden Gote scars: standardized overhangs for promoter, RBS, CDS and terminator]]  
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[[File:T--Valencia_UPV_TU_UPV2018.png|500px|thumb|none|alt=TU.|Figure 1. Assembled transcriptional unit. Golden Gate scars: standardized overhangs for promoter, RBS, CDS and terminator]]  
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It is a composite part of our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection#com '''BioArt DNA toolkit palette: Printone '''], as it can be used to express a green color with medium intensity.
  
 
This transcriptional unit is composed of the following standardized parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:  
 
This transcriptional unit is composed of the following standardized parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:  
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<ul>
 
<ul>
 
<li></html>[https://parts.igem.org/Part:BBa_K2656005 BBa_K2656005]: the [https://parts.igem.org/Part:J23102 J23102] constitutive promoter in its Golden Braid compatible version <html></li>
 
<li></html>[https://parts.igem.org/Part:BBa_K2656005 BBa_K2656005]: the [https://parts.igem.org/Part:J23102 J23102] constitutive promoter in its Golden Braid compatible version <html></li>
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<li></html>[https://parts.igem.org/Part:BBa_K2656009 BBa_K2656009]: the [https://parts.igem.org/Part:BBa_J61100 J61100]  strong ribosome biding site in its Golden Braid compatible version <html></li>
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<li></html>[https://parts.igem.org/Part:BBa_K2656009 BBa_K2656009]: the [https://parts.igem.org/Part:BBa_B0030 B0030]  strong ribosome biding site in its Golden Braid compatible version <html></li>
 
<li></html> [https://parts.igem.org/Part:BBa_K2656022 BBa_K2656022]: the [https://parts.igem.org/Part:BBa_K2656022 BBa_K2656022]  GFPmut3b sequence in its Golden Braid standardized version <html></li>
 
<li></html> [https://parts.igem.org/Part:BBa_K2656022 BBa_K2656022]: the [https://parts.igem.org/Part:BBa_K2656022 BBa_K2656022]  GFPmut3b sequence in its Golden Braid standardized version <html></li>
 
<li></html> Terminator  [https://parts.igem.org/Part:BBa_K2656026 BBa_K2656026]: the [https://parts.igem.org/Part:BBa_B0015 B0015] transcriptional terminator in its Golden Braid compatible version<html></li>
 
<li></html> Terminator  [https://parts.igem.org/Part:BBa_K2656026 BBa_K2656026]: the [https://parts.igem.org/Part:BBa_B0015 B0015] transcriptional terminator in its Golden Braid compatible version<html></li>
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</html>
 
</html>
  
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It has been used to characterize the constitutive [https://parts.igem.org/Part:BBa_K2656005 BBa_K2656005 promoter]. Following this [http://2018.igem.org/Team:Valencia_UPV/Modeling#http://2018.igem.org/Team:Valencia_UPV/Experiments#exp_protocol experimental protocol], we have obtained the parameters to valide our  [http://2018.igem.org/Team:Valencia_UPV/Modeling#models constitutive model] and so the results of the next graph have been obtained after an [http://2018.igem.org/Team:Valencia_UPV/Modeling#optimization optimization] and decision-making process, in which the optimal parameters have been selected and the constituent model for each objective has been simulated and compared with the experimental data.
 
  
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[[File:|900px|thumb|none|alt=Promoter experiment.|Figure 1. Promoter expression experiment with composite parts BBa_K2656105, BBa_K2656106 and BBa_K2656107]]
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This transcriptional unit has been used to characterize the constitutive [https://parts.igem.org/Part:BBa_K2656005 BBa_K2656005 promoter]. Following this [http://2018.igem.org/Team:Valencia_UPV/Modeling#http://2018.igem.org/Team:Valencia_UPV/Experiments#exp_protocol experimental protocol], we have obtained the parameters to valide our  [http://2018.igem.org/Team:Valencia_UPV/Modeling#models constitutive model] and so the results of the next graph have been obtained after an [http://2018.igem.org/Team:Valencia_UPV/Modeling#optimization optimization] and decision-making process, in which the optimal parameters have been selected and the constituent model for each objective has been simulated and compared with the experimental data.
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[[File:T--Valencia_UPV_promoter_UPV2018.png|900px|thumb|none|alt=Promoter experiment.|Figure 1. Promoter expression experiment with composite parts BBa_K2656105, BBa_K2656106 and BBa_K2656107]]
  
 
{|class='wikitable'
 
{|class='wikitable'
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|colspan=4|Table 1. Optimized parameters for the constitutive model with TU BBa_K2656106.
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|colspan=4|Table 1. Optimized parameters for the BBa_K2656106.
 
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|'''Parameter'''
 
|'''Parameter'''
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|Constitutive transcription rate CR
 
|Constitutive transcription rate CR
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|CR = 539.5 min-1
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|CR = 539.5 min-1
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| Dilution rate μ
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|μ = 0.0072 min-1
 
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|Dilution rate μ
 
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| μ = 0.0058 min-1
 
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| PoI degradation rate
 
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| Promoter Experiment: dp =
 
  
 
|}
 
|}
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It is also a composite part of our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection#com '''BioArt DNA toolkit palette: Printone '''], as it can be used to express a green color with medium intensity.
 
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Latest revision as of 01:50, 18 October 2018

GFP transcriptional unit 2

Transcriptional unit assembled with a one-pot [http://2018.igem.org/Team:Valencia_UPV/Design Level 1] Golden Gate reaction using BsaI type IIS endonuclease (Figure 1).

TU.
Figure 1. Assembled transcriptional unit. Golden Gate scars: standardized overhangs for promoter, RBS, CDS and terminator


It is a composite part of our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection#com BioArt DNA toolkit palette: Printone ], as it can be used to express a green color with medium intensity.

This transcriptional unit is composed of the following standardized parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:


This transcriptional unit has been used to characterize the constitutive BBa_K2656005 promoter. Following this [http://2018.igem.org/Team:Valencia_UPV/Modeling#http://2018.igem.org/Team:Valencia_UPV/Experiments#exp_protocol experimental protocol], we have obtained the parameters to valide our [http://2018.igem.org/Team:Valencia_UPV/Modeling#models constitutive model] and so the results of the next graph have been obtained after an [http://2018.igem.org/Team:Valencia_UPV/Modeling#optimization optimization] and decision-making process, in which the optimal parameters have been selected and the constituent model for each objective has been simulated and compared with the experimental data.

Promoter experiment.
Figure 1. Promoter expression experiment with composite parts BBa_K2656105, BBa_K2656106 and BBa_K2656107
Table 1. Optimized parameters for the BBa_K2656106.
Parameter Value
Constitutive transcription rate CR CR = 539.5 min-1
Dilution rate μ μ = 0.0072 min-1


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]