Difference between revisions of "Part:BBa K2656004"

Revision as of 22:13, 14 October 2018

Constitutive promoter J23106

Part BBa_K2656004 is the constitutive low promoter BBa_J23106 adapted into the Golden Braid 3.0. grammar. Thus, it is compatible with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/Design GoldenBraid] assembly methods.

It can be combined with other GB compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units in a BsaI Type IIS one-pot reaction with the [http://2018.igem.org/Team:Valencia_UPV/Protocols Golden Gate assembly protocol].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 11
Illegal NheI site found at 34
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 Part BBa_K2656004 is the constitutive low promoter [https://parts.igem.org/Part:BBa_J23106 BBa_J23106] adapted into the Golden Braid 3.0. grammar. Thus, it is compatible with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/Design GoldenBraid] assembly methods.
-It can be combined with other GB compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units in a BsaI Type IIS one-pot reaction by following the [http://2018.igem.org/Team:Valencia_UPV/Protocols Golden Gate assembly protocol].
+It can be combined with other GB compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units in a BsaI Type IIS one-pot reaction with the [http://2018.igem.org/Team:Valencia_UPV/Protocols Golden Gate assembly protocol].
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