Difference between revisions of "Part:BBa K2656201"

 
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<partinfo>BBa_K2656201 short</partinfo>
 
<partinfo>BBa_K2656201 short</partinfo>
  
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This part is a transcriptional unit including: BBa_K2656004 (GB 3 version of J23106 promoter), BBa_K2656009 (GB 3 version of B0030 rbs), BBa_K2656014 (GB 3 version of E1010 cds,mRFP1) and BBa_K2656026 (GB 3 version of B0015 terminator).
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This part is a transcriptional unit including:  
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In addition, this part is flanked upstream with the sequence ggagtgagacg and downstream with the sequence cgtctcagtca. These sequenses include a BsmbI recongition site and the appropriate resulting sticky end to accept an GB 3 Alpha1 construction.  
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<html>
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This construction can be used to subclone a composite GB3 compatible part into the BB backbone pSB1C3 as it is. Morover, it can be subcloned into any Biobrick compatible backbone to give the new plasmid the same ability.  
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<ul>
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<li></html>[https://parts.igem.org/Part:BBa_K2656004 BBa_K2656004 (GoldenBraid 3.0. version of J23106 promoter)]<html></li>
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<li></html> [https://parts.igem.org/Part:BBa_K2656009 BBa_K2656009 (GoldenBraid 3.0. version of B0030 RBS)]</li>
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<li></html>[https://parts.igem.org/Part:BBa_K2656014 BBa_K2656014 (GoldenBraid 3.0. version of E1010 CDS, mRFP1)]<html></li>
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<li></html>[https://parts.igem.org/Part:BBa_K2656026 BBa_K2656026 (GoldenBraid 3.0. version of B0015 terminator]<html></li>
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</ul>
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</html>
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In addition, this part is flanked upstream with the sequence ggagtgagacg and downstream with the sequence cgtctcagtca. These sequenses include a BsmbI recongition site and the appropriate resulting sticky end to accept an GB 3 alpha1 insert.  
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This construction has been ligated into the BB backbone pSB1C3, so designing the [https://parts.igem.org/Part:BBa_K2656200  BBa_K2656200 (pBioBrickator vector)]. Thus, in this BB compatible vector, BBa_K265620 acts as a selection marker to carry out the red-white screening while subcloning a composite GoldenBraid construction into the pSB1C3 vector. Moreover, this selection marker part can also be subcloned into any other Biobrick compatible backbone to give the new plasmid the same ability to accept GoldenBraid transcriptional units.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 23:12, 13 October 2018


Briobrickation part

This part is a transcriptional unit including:

In addition, this part is flanked upstream with the sequence ggagtgagacg and downstream with the sequence cgtctcagtca. These sequenses include a BsmbI recongition site and the appropriate resulting sticky end to accept an GB 3 alpha1 insert. This construction has been ligated into the BB backbone pSB1C3, so designing the BBa_K2656200 (pBioBrickator vector). Thus, in this BB compatible vector, BBa_K265620 acts as a selection marker to carry out the red-white screening while subcloning a composite GoldenBraid construction into the pSB1C3 vector. Moreover, this selection marker part can also be subcloned into any other Biobrick compatible backbone to give the new plasmid the same ability to accept GoldenBraid transcriptional units. Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 18
    Illegal NheI site found at 41
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 627
    Illegal AgeI site found at 739
  • 1000
    COMPATIBLE WITH RFC[1000]