Difference between revisions of "Part:BBa K2694000"
| (One intermediate revision by the same user not shown) | |||
| Line 3: | Line 3: | ||
<partinfo>BBa_K2694000 short</partinfo> | <partinfo>BBa_K2694000 short</partinfo> | ||
| − | + | The esterase in this circuit is an enzyme from Pseudomonas aeruginosa. The function of the esterase is to cleave ester bonds, such as those found in triglycerides. The production of the protein is under the control of a pLac promoter (<bbpart>BBa_R0011</bbpart>), with a strong ribosome binding site (<bbpart>BBa_B0034</bbpart>). At the end of the circuit there is a double terminator (<bbpart>BBa_B0010</bbpart> and <bbpart>BBa_B0012</bbpart>). The circuit was designed by the 2018 NDC-HighRiverAB team to produce the esterase protein for testing purposes. Our team introduced this circuit to DH5α and tested its activity towards two different substrates (4-nitrophenyl palmitate and 4-nitrophenyl octanoate). | |
| + | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 00:19, 12 October 2018
EstA generator under control of pLac promoter
The esterase in this circuit is an enzyme from Pseudomonas aeruginosa. The function of the esterase is to cleave ester bonds, such as those found in triglycerides. The production of the protein is under the control of a pLac promoter (BBa_R0011), with a strong ribosome binding site (BBa_B0034). At the end of the circuit there is a double terminator (BBa_B0010 and BBa_B0012). The circuit was designed by the 2018 NDC-HighRiverAB team to produce the esterase protein for testing purposes. Our team introduced this circuit to DH5α and tested its activity towards two different substrates (4-nitrophenyl palmitate and 4-nitrophenyl octanoate).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 296
Illegal BglII site found at 443 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 226
Illegal NgoMIV site found at 682
Illegal NgoMIV site found at 820
Illegal NgoMIV site found at 1360
Illegal NgoMIV site found at 1729 - 1000COMPATIBLE WITH RFC[1000]