Difference between revisions of "Part:BBa K2549039"

Revision as of 08:50, 11 October 2018

VP64-dNLS-ZF21.16

This part is one of the downstream elements of our amplifier. It is constructed by fusing VP64 (Part:BBa_K2549057), dNLS (Part:BBa_K2549056) and ZF21.16 (Part:BBa_K2549046), from N terminal to C terminal. VP64 is a tetrameric VP16 transcription activator which shows ultrahigh transcription activation function. dNLS, which is short for destroyable nuclear location sequence, is able to guide the fusion protein to be located to the nucleus without TEV protease. It is cleaved and unable to guide the fusion protein when TEV protease exist. Thus when coexpressed with NLS-TEVp, it is cleaved and the transcription activation function cannot be conducted.

Biology

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 502
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2549039 short</partinfo>
-This part is one of the downstream elements of our amplifier. It is constructed by fusing VP64 ([[Part:BBa_K1982012]]), dNLS ([[Part:BBa_K2549056]]) and ZF21.16 ([[Part:BBa_K2549046]]), from N terminal to C terminal. VP64 is a tetrameric VP16 transcription activator which shows ultrahigh transcription activation function. dNLS, which is short for destroyable nuclear location sequence, is able to guide the fusion protein to be located to the nucleus without TEV protease. It is cleaved and unable to guide the fusion protein when TEV protease exist. Thus when coexpressed with NLS-TEVp, it is cleaved and the transcription activation function cannot be conducted.
+This part is one of the downstream elements of our amplifier. It is constructed by fusing VP64 ([[Part:BBa_K2549057]]), dNLS ([[Part:BBa_K2549056]]) and ZF21.16 ([[Part:BBa_K2549046]]), from N terminal to C terminal. VP64 is a tetrameric VP16 transcription activator which shows ultrahigh transcription activation function. dNLS, which is short for destroyable nuclear location sequence, is able to guide the fusion protein to be located to the nucleus without TEV protease. It is cleaved and unable to guide the fusion protein when TEV protease exist. Thus when coexpressed with NLS-TEVp, it is cleaved and the transcription activation function cannot be conducted.
 <!-- Add more about the biology of this part here -->