Difference between revisions of "Part:BBa K2549023"

 
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This part is one of the downstream elements of our amplifier. Zinc finger 21.16 ([[Part:BBa_K2549046]]) is a synthetic engineered DNA binding domain with high sequence specificity and high orthogonality with other zinc finger proteins. VP64 ([[Part:BBa_K1982012]]) is a tetrameric VP16 transcription activator which shows ultrahigh transcription activation function. A SV40NLS domain ([[Part:BBa_K2549054]]) is placed on the N terminal of VP64 to guide the fusion protein to enter in the nucleus. A G4S linker ([[Part:BBa_K2549053]]) is set between ZF21.16 and SV40NLS. This part can also be utilized by other iGEM teams as zinc finger-based transcription activators to construct their own genetic circuits.
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===Biology===
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====previous reported engineered zinc finger-based transcription factors====
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Jason J. Lohmueller et al has reported a tunable zinc finger-based transcription framework in mammalian cells<ref>A tunable zinc finger-based framework for Boolean logic computation in mammalian cells. Lohmueller JJ, Armel TZ, Silver PA. Nucleic Acids Res, 2012 Jun;40(11):5180-7  PMID: 22323524; DOI: 10.1093/nar/gks142</ref>.
  
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===Usage and Biology===
 
  
 
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Revision as of 04:53, 10 October 2018


ZF21.16-VP64

This part is one of the downstream elements of our amplifier. Zinc finger 21.16 (Part:BBa_K2549046) is a synthetic engineered DNA binding domain with high sequence specificity and high orthogonality with other zinc finger proteins. VP64 (Part:BBa_K1982012) is a tetrameric VP16 transcription activator which shows ultrahigh transcription activation function. A SV40NLS domain (Part:BBa_K2549054) is placed on the N terminal of VP64 to guide the fusion protein to enter in the nucleus. A G4S linker (Part:BBa_K2549053) is set between ZF21.16 and SV40NLS. This part can also be utilized by other iGEM teams as zinc finger-based transcription activators to construct their own genetic circuits.

Biology

previous reported engineered zinc finger-based transcription factors

Jason J. Lohmueller et al has reported a tunable zinc finger-based transcription framework in mammalian cells[1].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 304
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


  1. ↑ A tunable zinc finger-based framework for Boolean logic computation in mammalian cells. Lohmueller JJ, Armel TZ, Silver PA. Nucleic Acids Res, 2012 Jun;40(11):5180-7 PMID: 22323524; DOI: 10.1093/nar/gks142