Difference between revisions of "Part:BBa J45003"
| (2 intermediate revisions by the same user not shown) | |||
| Line 2: | Line 2: | ||
<partinfo>BBa_J45003 short</partinfo> | <partinfo>BBa_J45003 short</partinfo> | ||
| − | <partinfo>BBa_J45003</partinfo> encodes SAM jasmonic acid carboxyl methyltransferase derived from '' | + | <partinfo>BBa_J45003</partinfo> encodes SAM jasmonic acid carboxyl methyltransferase derived from ''JMT'' from ''Arabidopsis thaliana''. JMT catalyzes the conversion of jasmonic acid to methyl jasmonate. Methyl jasmonate has a jasmine smell. |
===Usage and Biology=== | ===Usage and Biology=== | ||
*1 mM jasmonic acid and 1 mM S-adenosyl methionine (SAM) | *1 mM jasmonic acid and 1 mM S-adenosyl methionine (SAM) | ||
*Seo ''et al.'' were able to get the enzyme, purified from ''E. coli'', to catalyze the jasmine-smelling reaction ''in vitro''. | *Seo ''et al.'' were able to get the enzyme, purified from ''E. coli'', to catalyze the jasmine-smelling reaction ''in vitro''. | ||
| − | *K<sub>m</sub>: 38.5 μM; K<sub>cat</ | + | *K<sub>m</sub>: 38.5 μM; K<sub>cat</sub>: 25 s<sup>−1</sup> |
<!-- --> | <!-- --> | ||
Latest revision as of 17:35, 10 March 2008
SAM:jasmonic acid carboxyl methyltransferase; converts jasmonic acid to jasmine odor
BBa_J45003 encodes SAM jasmonic acid carboxyl methyltransferase derived from JMT from Arabidopsis thaliana. JMT catalyzes the conversion of jasmonic acid to methyl jasmonate. Methyl jasmonate has a jasmine smell.
Usage and Biology
- 1 mM jasmonic acid and 1 mM S-adenosyl methionine (SAM)
- Seo et al. were able to get the enzyme, purified from E. coli, to catalyze the jasmine-smelling reaction in vitro.
- Km: 38.5 μM; Kcat: 25 s−1
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 717
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1162
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 613