Difference between revisions of "Part:BBa J45003"

 
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<partinfo>BBa_J45003 short</partinfo>
 
<partinfo>BBa_J45003 short</partinfo>
  
This ORF encodes the enzyme responsible for catalyzing the following reaction:
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<partinfo>BBa_J45003</partinfo> encodes SAM jasmonic acid carboxyl methyltransferase derived from ''JMT'' from ''Arabidopsis thaliana''.  JMT catalyzes the conversion of jasmonic acid to methyl jasmonate.  Methyl jasmonate has a jasmine smell.
Jasmonic Acid--->Methyl Jasmonate
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It is initially found in ''Arabidopsis thaliana'' but has been transferred successfully to the E. coli genome by Seo, et alThe product, methyl jasmonate has a jasmine smell to it.
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===Usage and Biology===
 
===Usage and Biology===
1 mM JA and 1 mM S-adenosyl methionine (SAM)
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*1 mM jasmonic acid and 1 mM S-adenosyl methionine (SAM)
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*Seo ''et al.'' were able to get the enzyme, purified from ''E. coli'', to catalyze the jasmine-smelling reaction ''in vitro''.
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*K<sub>m</sub>: 38.5 &mu;M; K<sub>cat</sub>: 25 s<sup>−1</sup>
  
 
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Latest revision as of 17:35, 10 March 2008

SAM:jasmonic acid carboxyl methyltransferase; converts jasmonic acid to jasmine odor

BBa_J45003 encodes SAM jasmonic acid carboxyl methyltransferase derived from JMT from Arabidopsis thaliana. JMT catalyzes the conversion of jasmonic acid to methyl jasmonate. Methyl jasmonate has a jasmine smell.

Usage and Biology

  • 1 mM jasmonic acid and 1 mM S-adenosyl methionine (SAM)
  • Seo et al. were able to get the enzyme, purified from E. coli, to catalyze the jasmine-smelling reaction in vitro.
  • Km: 38.5 μM; Kcat: 25 s−1

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 717
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1162
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 613