Difference between revisions of "Part:BBa J45003"

Revision as of 21:56, 9 March 2008

SAM:jasmonic acid carboxyl methyltransferase; converts jasmonic acid to jasmine odor

BBa_J45003 encodes SAM jasmonic acid carboxyl methyltransferase derived from JAMT from Arabidopsis thaliana. JAMT catalyzes the conversion of jasmonic acid to methyl jasmonate. Methyl jasmonate has a jasmine smell.

Usage and Biology

1 mM jasmonic acid and 1 mM S-adenosyl methionine (SAM)
Seo et al. were able to get the enzyme, purified from E. coli, to catalyze the jasmine-smelling reaction in vitro.
K_m: 38.5 μM; K_{cat</sup>: 25 s⁻¹}

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 717
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1162
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 613

@@ Line 2: / Line 2: @@
 <partinfo>BBa_J45003 short</partinfo>
-This ORF encodes the enzyme responsible for catalyzing the following reaction:
+<partinfo>BBa_J45003</partinfo> encodes SAM jasmonic acid carboxyl methyltransferase derived from ''JAMT'' from ''Arabidopsis thaliana''.  JAMT catalyzes the conversion of jasmonic acid to methyl jasmonate.  Methyl jasmonate has a jasmine smell.
-Jasmonic Acid--->Methyl Jasmonate
-It is initially found in ''Arabidopsis thaliana'' but has been transferred successfully to the E. coli genome by Seo, et al.  The product, methyl jasmonate has a jasmine smell to it.  For more information, see http://openwetware.org/wiki/IGEM:MIT/2006/Notebook/2006-6-6.
 ===Usage and Biology===
 *1 mM jasmonic acid and 1 mM S-adenosyl methionine (SAM)
-*Seo ''et al.'' were able to get the enzyme, purified from ''E. coli'', to catalyze the jasmine-smelling reaction in vitro.
+*Seo ''et al.'' were able to get the enzyme, purified from ''E. coli'', to catalyze the jasmine-smelling reaction ''in vitro''.
-*Km: 38.5 &mu;M; Kcat:25 S−1
+*K<sub>m</sub>: 38.5 &mu;M; K<sub>cat</sup>: 25 s<sup>−1</sup>
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