Difference between revisions of "Part:BBa J45003"
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It is initially found in ''Arabidopsis thaliana'' but has been transferred successfully to the E. coli genome by Seo, et al. The product, methyl jasmonate has a jasmine smell to it. For more information, see http://openwetware.org/wiki/IGEM:MIT/2006/Notebook/2006-6-6. | It is initially found in ''Arabidopsis thaliana'' but has been transferred successfully to the E. coli genome by Seo, et al. The product, methyl jasmonate has a jasmine smell to it. For more information, see http://openwetware.org/wiki/IGEM:MIT/2006/Notebook/2006-6-6. | ||
| − | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | 1 mM | + | *1 mM jasmonic acid and 1 mM S-adenosyl methionine (SAM) |
| + | *Seo ''et al.'' were able to get the enzyme, purified from ''E. coli'', to catalyze the jasmine-smelling reaction in vitro. | ||
| + | *Km: 38.5 μM; Kcat:25 S−1 | ||
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Revision as of 21:50, 9 March 2008
SAM:jasmonic acid carboxyl methyltransferase; converts jasmonic acid to jasmine odor
This ORF encodes the enzyme responsible for catalyzing the following reaction:
Jasmonic Acid--->Methyl Jasmonate
It is initially found in Arabidopsis thaliana but has been transferred successfully to the E. coli genome by Seo, et al. The product, methyl jasmonate has a jasmine smell to it. For more information, see http://openwetware.org/wiki/IGEM:MIT/2006/Notebook/2006-6-6.
Usage and Biology
- 1 mM jasmonic acid and 1 mM S-adenosyl methionine (SAM)
- Seo et al. were able to get the enzyme, purified from E. coli, to catalyze the jasmine-smelling reaction in vitro.
- Km: 38.5 μM; Kcat:25 S−1
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 717
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1162
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 613