Difference between revisions of "Part:BBa J45001:Experience"

Latest revision as of 21:49, 9 March 2008

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_J45001

Used in wintergreen odor production.

User Reviews

UNIQecb4298b3b0684a8-partinfo-00000000-QINU

•••••

Reshma Shetty

Preliminary smell tests indicated that BBa_J45001 produces a wintergreen odor in culture when supplemented with salicylic acid.

UNIQecb4298b3b0684a8-partinfo-00000003-QINU

@@ Line 4: / Line 4: @@
 ===Applications of BBa_J45001===
+*Used in wintergreen odor production.
 ===User Reviews===
-===Enzyme Activity===
-#E. coli-expressed SAMT protein catalyzes the formation of the volatile ester methyl salicylate from salicylic acid with a Km (SA) of 83 and a Km (SAM) of 3-4. It can also methylate benzoic acid to form methyl benzoate, but its K(m) value for benzoic acid is 1720 M (much larger). Kcat/Km for SA is 132 s-1 M-1, and activity with substrate benzoic acid is 45% of the activity with substrate salicylic acid. All other acids yield 0% activity.
 <!-- DON'T DELETE --><partinfo>BBa_J45001 StartReviews</partinfo>
 <!-- Template for a user review
@@ Line 21: / Line 18: @@
 |};
 <!-- End of the user review template -->
+{|width='80%' style='border:1px solid gray'
+|-
+|width='10%'|
+<partinfo>BBa_J45001 AddReview 5</partinfo><br>
+<I>[[User:Rshetty|Reshma Shetty]]</I>
+|width='60%' valign='top'|
+Preliminary smell tests indicated that <partinfo>BBa_J45001</partinfo> produces a wintergreen odor in culture when supplemented with salicylic acid.
+|}
 <!-- DON'T DELETE --><partinfo>BBa_J45001 EndReviews</partinfo>