Difference between revisions of "Part:BBa J45001:Design"

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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_J45001 short</partinfo>
 
<partinfo>BBa_J45001 short</partinfo>
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===Design Notes===
 
===Design Notes===
−
open reading frame= 9-1160
+
*No BioBrick restriction enzyme cut sites in coding region
 +
*Changed natural stop codon to TAA TAA
 +
*Constructed by PCR
  
−
===Source===
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====Forward primer====
 +
<code>5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA G'''AT GAC AAA ACA AAC ACA AAA G''' -3'</code>
  
−
Dutareva
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====Reverse primer====
 +
<code>5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TA'''c tct cgc ctt gtc atg gaa ata g''' -3'</code>
 +
 
 +
====Internal sequencing primer====
 +
<code>5'- gga atg ctg cta tat ttg gg -3'</code>
 +
 
 +
===Source===
 +
''SAMT'' gene from ''Antirrhinum majus'' (snapdragon flower) ([http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=23957317 GenBank AF515284])
  
 
===References===
 
===References===
 +
<biblio>
 +
#Negre-ArchBiochemBiophys-2002 pmid=12361714
 +
</biblio>
 +
 +
*[http://openwetware.org/wiki/IGEM:MIT/2006/Notebook/2006-6-6 MIT iGEM 2006 notebook entry]

Latest revision as of 21:41, 9 March 2008

SAM:salicylic acid carboxyl methyltransferase; converts salicylic acid to methyl salicylate (winter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 577
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 901
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 421
    Illegal NgoMIV site found at 425
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 471
    Illegal SapI.rc site found at 75


Design Notes

  • No BioBrick restriction enzyme cut sites in coding region
  • Changed natural stop codon to TAA TAA
  • Constructed by PCR

Forward primer

5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAT GAC AAA ACA AAC ACA AAA G -3'

Reverse primer

5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAc tct cgc ctt gtc atg gaa ata g -3'

Internal sequencing primer

5'- gga atg ctg cta tat ttg gg -3'

Source

SAMT gene from Antirrhinum majus (snapdragon flower) ([http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=23957317 GenBank AF515284])

References

<biblio>

  1. Negre-ArchBiochemBiophys-2002 pmid=12361714

</biblio>

  • [http://openwetware.org/wiki/IGEM:MIT/2006/Notebook/2006-6-6 MIT iGEM 2006 notebook entry]