Difference between revisions of "Part:BBa J45001"
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===Usage and Biology=== | ===Usage and Biology=== | ||
− | + | The culture medium of ''E. coli'' cells expressing ''SAMT'' contained methyl salicylate (2.1 μg/ml) when the growing medium was supplemented with 5 μg/ml salicylic acid, and methyl benzoate (0.86 μg/ml) when the growing medium was supplemented with 5 μg/ml benzoic acid. | |
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Revision as of 21:37, 9 March 2008
SAM:salicylic acid carboxyl methyltransferase; converts salicylic acid to methyl salicylate (winter
BBa_J45001 encodes SAM salicylic acid carboxyl methyltransferase I derived from SAMT from Antirrhinus majus (snapdragon). SAMT catalyzes the conversion of salicylic acid to methyl salicylate. Methyl salicylate has a wintergreen smell.
Usage and Biology
The culture medium of E. coli cells expressing SAMT contained methyl salicylate (2.1 μg/ml) when the growing medium was supplemented with 5 μg/ml salicylic acid, and methyl benzoate (0.86 μg/ml) when the growing medium was supplemented with 5 μg/ml benzoic acid.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 577
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 901
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 421
Illegal NgoMIV site found at 425 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 471
Illegal SapI.rc site found at 75
Functional Parameters
ec_num | none |
kegg | none |
protein | SAMT |
swisspro | Q8H6N2 |