Difference between revisions of "Part:BBa J45002"

Latest revision as of 21:35, 9 March 2008

SAM:benzoic acid carboxyl methyltransferase; converts benzoic acid to methyl benzoate (floral odor)

BBa_J45002 encodes SAM benzoic acid carboxyl methyltransferase I derived from BAMT from Antirrhinus majus (snapdragon). BAMT catalyzes the conversion of benzoic acid to methyl benzoate. Methyl benzoate has a floral smell.

Usage and Biology

Enzyme is active as a dimer.
There are two methionine codons at the begnning of the BAMT cDNA. The BAMT resulting from amplification from the second methionine shows a 2.4 times higher specific activity than from the first methionine in protein purified from cell lysate; on the other hand, BAMT purified from E. coli shows no difference in specific activity (from both Met codons). The K_m and k_cat are the same from both codons in E. coli.
Inhibition by SAH was competitive with respect to SAM and noncompetitive with respect to benzoic acid. SAM appears to be the first substrate to bind to the enzyme. Methyl benzoate is released first and SAH last.
BAMT activity may be regulated by the intracellular SAM/SAH concentration ratio rather than benzoic acid availability.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 970
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 654
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Functional Parameters

ec_num	2.1.1.-
kegg	none
protein	BAMT
swisspro	Q9FYZ9

@@ Line 2: / Line 2: @@
 <partinfo>BBa_J45002 short</partinfo>
-<partinfo>BBa_J45002</partinfo> encodes SAM benzoic acid methyltransferase I derived from ''BAMT'' from ''Antirrhinus majus'' (snapdragon). BAMT catalyzes the conversion of benzoic acid to methyl benzoate.  Methyl benzoate has a floral smell.
+<partinfo>BBa_J45002</partinfo> encodes SAM benzoic acid carboxyl methyltransferase I derived from ''BAMT'' from ''Antirrhinus majus'' (snapdragon). BAMT catalyzes the conversion of benzoic acid to methyl benzoate.  Methyl benzoate has a floral smell.
 ===Usage and Biology===
-*Genbank accession number(s): AF198492.
 *Enzyme is active as a dimer.
-*There are two Met codons at the begnning of the BAMT cDNA.  The BAMT resulting from amplification from the second Met shows a 2.4 times higher specific activity than from the first Met in protein purified from cell lysate; on the other hand, BAMT purified from ''E. coli'' shows no difference in specific activity (from both Met codons).  The K<sub>m</sub> and k<sub>cat</sub> are the same from both codons in ''E. coli''.
+*There are two methionine codons at the begnning of the BAMT cDNA.  The BAMT resulting from amplification from the second methionine shows a 2.4 times higher specific activity than from the first methionine in protein purified from cell lysate; on the other hand, BAMT purified from ''E. coli'' shows no difference in specific activity (from both Met codons).  The K<sub>m</sub> and k<sub>cat</sub> are the same from both codons in ''E. coli''.
-*Inhibition by SAH was competitive with respect to SAM and noncompetitive with respect to BA.  SAM appears to be the first substrate to bind to the enzyme. Methyl benzoate is released first and SAH last.
+*Inhibition by SAH was competitive with respect to SAM and noncompetitive with respect to benzoic acid.  SAM appears to be the first substrate to bind to the enzyme. Methyl benzoate is released first and SAH last.
-*BAMT activity may be regulated by the intracellular SAM/SAH concentration ratio rather than BA availability.
+*BAMT activity may be regulated by the intracellular SAM/SAH concentration ratio rather than benzoic acid availability.
 <span class='h3bb'>Sequence and Features</span>