Difference between revisions of "Part:BBa J45999"

Latest revision as of 20:06, 9 March 2008

E. coli strain YYC912; Odor-free chassis

Escherichia coli strain [http://cgsc2.biology.yale.edu/Strain.php?ID=64826 YYC912] (BBa_J45999) is a biosafety level 1 organism derived from E. coli K12. E. coli strain YYC912 is an odor-free chassis because it produces no detectable indole. Indole is responsible for the foul smell of E. coli.

Usage and Biology

CGSC#: 7602
Strain Designation
- Designation: YYC912
- Source Person: Cronan & Chang
- Choice: 1
Sex(Hfr,F+,F-,or F'): F-
Mutations
- DE(argF-lac)169
- LAM-
- poxB15::lacZ::CmR (Note chloramphenicol resistance)
- IN(rrnD-rrnE)1
- rph-1
- tnaA5
No. of Muts Carried: 6
Special Uses: lac fusion-stat.phase promoter
Has_Partner_Isogenic_WRT: poxB15::lacZ::CmR
Isogenic_Partner_Is: ZK126
References Chang-1994
ID#: 64826

Chang-1994 pmid=8022274

</biblio>

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_J45999 short</partinfo>
-''Escherichia coli'' strain [http://cgsc2.biology.yale.edu/Strain.php?ID=64826 YYC912].  Biosafety level 1 organism derived from ''Escherichia coli'' K12.
+''Escherichia coli'' strain [http://cgsc2.biology.yale.edu/Strain.php?ID=64826 YYC912] (<partinfo>BBa_J45999</partinfo>) is a biosafety level 1 organism derived from ''E. coli'' K12.  ''E. coli'' strain YYC912 is an odor-free chassis because it produces no detectable indole.  Indole is responsible for the foul smell of ''E. coli''.
-BBa_J45999 has a mutation in the ''tnaA'' gene which encodes a tryptophanase which converts tryptophan to indole.  Indole is responsible for the foul stench of ''E. coli'' so ''tnaA'' mutants can have reduced indole levels and thus reduced foul odor.
 ===Usage and Biology===