Difference between revisions of "Part:BBa J45999"

Revision as of 19:49, 9 March 2008

E. coli strain YYC912; Odor-free chassis

Escherichia coli strain [http://cgsc2.biology.yale.edu/Strain.php?ID=64826 YYC912]. Biosafety level 1 organism derived from Escherichia coli K12.

BBa_J45999 has a mutation in the tnaA gene which encodes a tryptophanase which converts tryptophan to indole. Indole is responsible for the foul stench of E. coli so tnaA mutants can have reduced indole levels and thus reduced foul odor.

Usage and Biology

• CGSC#: 7602
• Strain Designation
  • Designation: YYC912
  • Source Person: Cronan & Chang
  • Choice: 1
• Sex(Hfr,F+,F-,or F'): F-
• Mutations
  • DE(argF-lac)169
  • LAM-
  • poxB15::lacZ::CmR -- Note chloramphenicol resistance
  • IN(rrnD-rrnE)1
  • rph-1
  • tnaA5
• No. of Muts Carried: 6
• Special Uses: lac fusion-stat.phase promoter
• Has_Partner_Isogenic_WRT: poxB15::lacZ::CmR
• Isogenic_Partner_Is: ZK126
• References
  • Chang, Y.-Y., A.Y. Wang, J.E. Cronan 1994. Expression of Escherichia coli pyruvate oxidase (PoxB) depends on the sigma factor encoded by the rpoS(katF) gene. Mol.Microbiol. 11:1019-1028
• ID#: 64826

Sequence and Features