Difference between revisions of "Part:BBa J45250"
 
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<partinfo>BBa_J45270 short</partinfo>
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<partinfo>BBa_J45250 short</partinfo>
  
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This part is an ATF1-1148 protein generator under control by the osmY stationary phase promoter (<partinfo>J45993</partinfo>).  It is almost identical to <partinfo>J45270</partinfo>, except that:
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The stationary phase dependent banana odor generator (<partinfo>BBa_J45250</partinfo>) catalyzes the conversion of the precursor isoamyl alcohol to the odor isoamyl acetate that has a banana smell.  The biosynthetic device is composed of two transcriptional devices: a constitutive transcription source (<partinfo>BBa_J45992</partinfo>) and an odor enzyme generator (<partinfo>BBa_J45199</partinfo>). Odor enzyme generators take as input a transcriptional signal and produce as output an enzyme that catalyzes production of an odor from a chemical precursor. 
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#The promoter is the truncated version of osmY and  
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#it uses the B0030 RBS instead of B0032 (which is in <partinfo>J45270</partinfo>).
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[[Image:BBa_J45250.png|600px|center]]
  
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===Usage and Biology===
 
===Usage and Biology===
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*Supplement cultures with 5 mM isoamyl alcohol to produce banana odor.
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*See <partinfo>BBa_J45014</partinfo> for details.
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_J45270 parameters</partinfo>
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<partinfo>BBa_J45250 parameters</partinfo>
 
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Latest revision as of 17:59, 8 March 2008

Stationary-phase-dependent banana odor generator

The stationary phase dependent banana odor generator (BBa_J45250) catalyzes the conversion of the precursor isoamyl alcohol to the odor isoamyl acetate that has a banana smell. The biosynthetic device is composed of two transcriptional devices: a constitutive transcription source (BBa_J45992) and an odor enzyme generator (BBa_J45199). Odor enzyme generators take as input a transcriptional signal and produce as output an enzyme that catalyzes production of an odor from a chemical precursor.

BBa J45250.png

Usage and Biology

  • Supplement cultures with 5 mM isoamyl alcohol to produce banana odor.
  • See BBa_J45014 for details.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 327
    Illegal BamHI site found at 1654
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 754
    Illegal SapI.rc site found at 1762