Difference between revisions of "Part:BBa J45200:Design"

(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
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None.
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*Eliminated EcoRI site at base pair 412 by changing base pair 414 from A to G
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*forward primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA G<b>AT GAA TGA AAT CGA TGA G</b> -3'
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*new forward primer: 5' - GTT TCT TCG AAT TCG CGG CCG CTT CTA G<b>AT GAA TGA AAT CGA TGA GAA AAA TC</b> -3'
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*new reverse primer: 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT <b>TAa ggg cct aaa agg aga gct ttg</b> -3'
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*forward mutagenesis primer: 5' GAA GCA AAT ATT AGA AGA GTT CAA AAA TAG TAA GGG 3'
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*reverse mutagenesis primer: 5' CCC TTA CTA TTT TTG AAC TCT TCT AAT ATT TGC TTC 3'
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[[Image:J45200.jpg]]
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===Source===
 
===Source===
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Source: ''S. cerevisiae'', chromosome XV
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<partinfo>BBa_R0040</partinfo> and <partinfo>BBa_J45199</partinfo>.
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Sequence obtained from Genbank, accession number [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=Nucleotide&dopt=GenBank&val=1420813 Z75285]
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===References===
 
===References===
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<biblio>
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See [[Part:BBa_J45014:Design#References]].
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#*Horton03 pmid=12937998
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</biblio>
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Latest revision as of 06:36, 8 March 2008

Banana odor generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 182
    Illegal BamHI site found at 1509
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 609
    Illegal SapI.rc site found at 1617

Design Notes

None.

Source

BBa_R0040 and BBa_J45199.

References

See Part:BBa_J45014:Design#References.