Difference between revisions of "Part:BBa J45200:Design"
 
(One intermediate revision by one other user not shown)
Line 5: Line 5:
  
 
===Design Notes===
 
===Design Notes===
−
 
+
None.
−
*Eliminated EcoRI site at base pair 412 by changing base pair 414 from A to G
+
−
*forward primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA G<b>AT GAA TGA AAT CGA TGA G</b> -3'
+
−
*new forward primer: 5' - GTT TCT TCG AAT TCG CGG CCG CTT CTA G<b>AT GAA TGA AAT CGA TGA GAA AAA TC</b> -3'
+
−
*new reverse primer: 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT <b>TAa ggg cct aaa agg aga gct ttg</b> -3'
+
−
 
+
−
*forward mutagenesis primer: 5' GAA GCA AAT ATT AGA AGA GTT CAA AAA TAG TAA GGG 3'
+
−
*reverse mutagenesis primer: 5' CCC TTA CTA TTT TTG AAC TCT TCT AAT ATT TGC TTC 3'
+
  
 
===Source===
 
===Source===
−
Source: ''S. cerevisiae'', chromosome XV
+
<partinfo>BBa_R0040</partinfo> and <partinfo>BBa_J45199</partinfo>.
−
 
+
−
Sequence obtained from Genbank, accession number [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=Nucleotide&dopt=GenBank&val=1420813 Z75285]
+
  
 
===References===
 
===References===
−
<biblio>
+
See [[Part:BBa_J45014:Design#References]].
−
#*Horton03 pmid=12937998
+
−
</biblio>
+

Latest revision as of 06:36, 8 March 2008

Banana odor generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 182
    Illegal BamHI site found at 1509
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 609
    Illegal SapI.rc site found at 1617

Design Notes

None.

Source

BBa_R0040 and BBa_J45199.

References

See Part:BBa_J45014:Design#References.