Difference between revisions of "Part:BBa J45200"

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<partinfo>BBa_J45200 short</partinfo>
 
<partinfo>BBa_J45200 short</partinfo>
  
Protein generator producing the protein ATF1 under control of the LacI repressor.  Thus, in a LacI<sup>-</sup> ''E. coli'' strain, ATF1 is continually produced.  ATFI encodes the enzyme alcohol acetyltransferase I (AATase I), which produces acetate esters from acetyl CoA and alcohols (including isoamyl acetate from isoamyl alcohol)This part is almost identical to <partinfo>J45220</partinfo>  except that it uses the B0030 RBS (instead of B0032 as in <partinfo>J45220</partinfo>).  The Banana Generating Device (BGD) function thus far has been to convert isoamyl alcohol into isoamyl acetate (a banana-scented compound).
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The banana odor generator (<partinfo>BBa_J45200</partinfo>) catalyzes the conversion of the precursor isoamyl alcohol to the odor isoamyl acetate that has a banana smellThe biosynthetic device is composed of two transcriptional devices: a constitutive transcription source (<partinfo>BBa_R0040</partinfo) and an odor enzyme generator (<partinfo>BBa_J45199</partinfo).  Odor enzyme generators take as input a transcriptional signal and produce as output an enzyme that catalyzes production of an odor from a chemical precursor.
  
[[Image:J45200.jpg]]
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[[Image:J45200.jpg|center]]
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===Usage and Biology===
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See <partinfo>BBa_J45014</partinfo> for details.
  
  

Revision as of 06:33, 8 March 2008

Banana odor generator

The banana odor generator (BBa_J45200) catalyzes the conversion of the precursor isoamyl alcohol to the odor isoamyl acetate that has a banana smell. The biosynthetic device is composed of two transcriptional devices: a constitutive transcription source (No part name specified with partinfo tag. for details.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 182
    Illegal BamHI site found at 1509
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 609
    Illegal SapI.rc site found at 1617