Difference between revisions of "Part:BBa Q04401"

Latest revision as of 01:19, 8 March 2008

TetR-derived transcriptional inverter

Transcriptional inverter based on tetracycline repressor and a cognate promoter.

Usage and Biology

BBa_Q04401 is a modified version of the tetR based inverter BBa_Q04400 with a single base mutation in the ribosome binding site: AAAGAGGAGAAA to AAAGAGGGGAAA.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_Q04401 short</partinfo>
-This is a modified version of the tetR inverter <bbpart>Q04400</bbpart> with a single base switch in the RBS:
+Transcriptional inverter based on tetracycline repressor and a cognate promoter.
-AAAGAGGAGAAA to AAAGAGGGGAAA
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+<partinfo>BBa_Q04401</partinfo> is a modified version of the tetR based inverter <partinfo>Q04400</partinfo> with a single base mutation in the ribosome binding site: <code>AAAGAGGAGAAA</code> to <code>AAAGAGGGGAAA</code>.
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_Q04401 SequenceAndFeatures</partinfo>
-[[Image:Q04401.JPG|thumb|500px|left|Transfer curve for Q04401 using the Screening Plasmid (<bbpart>pSB1A10</bbpart>)]]
-<br style="clear:both" />
 <!-- Uncomment this to enable Functional Parameter display
@@ Line 20: / Line 15: @@
 <partinfo>BBa_Q04401 parameters</partinfo>
 <!-- -->
-===Reuse===
-*This inverter was successfully reused by the [http://openwetware.org/wiki/IGEM:MIT/2006 2006 MIT iGEM team].
-*'''[[User:Jasonk|Jasonk]] 02:44, 23 February 2008 (EST):'''In general, I think it's more likely to work when used on a high-copy (1 series) plasmid.  I've tried it on a 3 series, using <bbpart>F2621</bbpar> as the induction system and did not see flipping of the inverter.</bbpart>