Difference between revisions of "Part:BBa Q04401"

 
(11 intermediate revisions by one other user not shown)
Line 2: Line 2:
 
<partinfo>BBa_Q04401 short</partinfo>
 
<partinfo>BBa_Q04401 short</partinfo>
  
Currently entered as a placeholder. This is a modified version of the tetR inverter Q04400 with a single base switch in the RBS:
+
Transcriptional inverter based on tetracycline repressor and a cognate promoter.
AAAGAGGAGAAA to AAAGAGGGGAAA
+
  
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
<partinfo>BBa_Q04401</partinfo> is a modified version of the tetR based inverter <partinfo>Q04400</partinfo> with a single base mutation in the ribosome binding site: <code>AAAGAGGAGAAA</code> to <code>AAAGAGGGGAAA</code>.
  
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_Q04401 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_Q04401 SequenceAndFeatures</partinfo>
 
 
[[Image:Q04401.JPG|thumb|500px|left|Transfer curve for Q04401 using the Screening Plasmid (<bbpart>pSB1A10</bbpart>)]]
 
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 01:19, 8 March 2008

TetR-derived transcriptional inverter

Transcriptional inverter based on tetracycline repressor and a cognate promoter.

Usage and Biology

BBa_Q04401 is a modified version of the tetR based inverter BBa_Q04400 with a single base mutation in the ribosome binding site: AAAGAGGAGAAA to AAAGAGGGGAAA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]