Difference between revisions of "Part:BBa J45996:Experience"

 
 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
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===Applications of BBa_J45996===
 
===Applications of BBa_J45996===
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Exponential phase dependent fluorescence.
  
 
===User Reviews===
 
===User Reviews===
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<partinfo>BBa_J45996 AddReview 5</partinfo><br>
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<I>[[User:Rshetty|Reshma Shetty]]</I>
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<partinfo>BBa_J45996</partinfo> produced fluorescence only in exponential phase. 
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==Characterization==
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===Transcriptional control of GFP generator===
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[Note: <partinfo>BBa_J45996</partinfo> is a composite part of <partinfo>BBa_J45994</partinfo> and <partinfo>BBa_E0840</partinfo>.]
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{{:iGEM:MIT/2006/Transcriptional control devices}}
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Latest revision as of 01:11, 8 March 2008

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_J45996

Exponential phase dependent fluorescence.

User Reviews

UNIQ52fd42c501c13656-partinfo-00000000-QINU

•••••

Reshma Shetty

BBa_J45996 produced fluorescence only in exponential phase.

UNIQ52fd42c501c13656-partinfo-00000003-QINU

Characterization

Transcriptional control of GFP generator

[Note: BBa_J45996 is a composite part of BBa_J45994 and BBa_E0840.]

Growth phase dependent transcriptional control devices
We successfully designed, constructed and tested transcriptional control devices for constitutive, stationary phase dependent and exponential phase dependent protein production (A-C). To test and verify function of our three transcriptional control devices, we assembled each control device with the GFP protein generator BBa_E0840 and monitored the fluorescence of E. coli cultures with each device over time. For each device, we plot the change in fluorescence per unit time (normalized GFP synthesis rate) versus the cell density (OD600nm) (D). The constitutive transcriptional control device produced a high GFP synthesis rate irrespective of cell density. The stationary phase transcriptional control device produced a low initial GFP synthesis rate which increased with culture cell density. The exponential phase transcriptional control device produced an initially high GFP synthesis rate which dropped off as cell density increased. Data shown are averages of triplicate measurements of cultures grown from three individual colonies of each device. Error bars are the standard deviation of the three individual cultures.