Difference between revisions of "Part:BBa J45992:Design"

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===Design Notes===
 
===Design Notes===
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*We considered different designs for stationary phase regulation of odor production.  All designs focused on using transcriptional control devices to regulate the odor enzyme generators and thus odor production.  One candidate design for stationary phase regulation was to use a transcriptional promoter that is only active in stationary phase.  Several ''E. coli'' promoters that are primarily active in stationary phase have been previously characterized <cite>Schellhorn-1998, Vijayakumar-2004</cite>.  Thus, we could evaluate various stationary phase promoters as potential growth phase dependent transcriptional control devices.
 
*Constructed by PCR
 
*Constructed by PCR
  

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Design Notes

  • We considered different designs for stationary phase regulation of odor production. All designs focused on using transcriptional control devices to regulate the odor enzyme generators and thus odor production. One candidate design for stationary phase regulation was to use a transcriptional promoter that is only active in stationary phase. Several E. coli promoters that are primarily active in stationary phase have been previously characterized Schellhorn-1998, Vijayakumar-2004. Thus, we could evaluate various stationary phase promoters as potential growth phase dependent transcriptional control devices.
  • Constructed by PCR

Template

Escherichia coli strain MG1655

Forward Primer

5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GCT GGC ACA GGA ACG TTA TC - 3'

Reverse Primer

5'-GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TGT TAA ATA TAG ATC ACA ATT TTG AAA CCG- 3'

Source

  • Promoter controlling expression of osmY from Escherichia coli (GenBank [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=453110 S67278])

References

<biblio>

  1. Yim-1994 pmid=8282684
  2. Schellhorn-1998 pmid=9829938
  3. Wise pmid=8631665
  4. Funabashi pmid=11879714
  5. Makinoshima pmid=11985708
  6. Yim-1992 pmid=1317380
  7. Hengge-Aronis-1993 pmid=8416901
  8. Vijaykumar-2004 pmid=15576800

</biblio>


Neidhart, Frederick C. Escherichia coli and Salmonella. Washington: ASM Press, 1996.