Difference between revisions of "Part:BBa J45992:Design"

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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_J45992 short</partinfo>
 
<partinfo>BBa_J45992 short</partinfo>
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===Design Notes===
 
===Design Notes===
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Primers used to PCR this part out of the ''E.coli'' genome:
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*Constructed by PCR
 +
 
 +
====Template====
 +
*''Escherichia coli'' strain MG1655
  
 
====Forward Primer====
 
====Forward Primer====
−
5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GCT GGC ACA GGA ACG TTA TC - 3'
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<code>5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GCT GGC ACA GGA ACG TTA TC - 3'</code>
  
 
====Reverse Primer====
 
====Reverse Primer====
−
5'-GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TGT TAA ATA TAG ATC ACA ATT TTG AAA CCG- 3'
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<code>5'-GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TGT TAA ATA TAG ATC ACA ATT TTG AAA CCG- 3'</code>
  
 
===Source===
 
===Source===
−
 
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*Promoter controlling expression of ''osmY'' from ''Escherichia coli'' (GenBank [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=453110 S67278])
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This part was PCRed out of E. coli's genome with the primers listed in the design notes section.
+
  
 
===References===
 
===References===
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</biblio>
 
</biblio>
  
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[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=453110 S67278]
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Neidhart, Frederick C. ''Escherichia coli and Salmonella''. Washington: ASM Press, 1996.
 
Neidhart, Frederick C. ''Escherichia coli and Salmonella''. Washington: ASM Press, 1996.

Revision as of 23:35, 7 March 2008

Full-length stationary phase osmY promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • Constructed by PCR

Template

  • Escherichia coli strain MG1655

Forward Primer

5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GCT GGC ACA GGA ACG TTA TC - 3'

Reverse Primer

5'-GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TGT TAA ATA TAG ATC ACA ATT TTG AAA CCG- 3'

Source

  • Promoter controlling expression of osmY from Escherichia coli (GenBank [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=453110 S67278])

References

<biblio>

  1. Yim-1994 pmid=8282684
  2. Schellhorn-1998 pmid=9829938
  3. Wise pmid=8631665
  4. Funabashi pmid=11879714
  5. Makinoshima pmid=11985708
  6. Yim-1992 pmid=1317380
  7. Hengge-Aronis-1993 pmid=8416901
  8. Vijaykumar-2004 pmid=15576800

</biblio>


Neidhart, Frederick C. Escherichia coli and Salmonella. Washington: ASM Press, 1996.