Difference between revisions of "Part:BBa J45992:Design"

(→‎Forward Primer)
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===References===
 
===References===
 
<biblio>
 
<biblio>
−
#Yim pmid=8282684
+
#Yim-1994 pmid=8282684
−
#Schellhorn pmid=9829938
+
#Schellhorn-1998 pmid=9829938
 
#Wise pmid=8631665
 
#Wise pmid=8631665
 
#Funabashi pmid=11879714
 
#Funabashi pmid=11879714
 
#Makinoshima pmid=11985708
 
#Makinoshima pmid=11985708
 +
#Yim-1992 pmid=1317380
 +
#Hengge-Aronis-1993 pmid=8416901
 +
#Vijaykumar-2004 pmid=15576800
 
</biblio>
 
</biblio>
  

Revision as of 23:27, 7 March 2008


Full-length stationary phase osmY promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers used to PCR this part out of the E.coli genome:

Forward Primer

5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GCT GGC ACA GGA ACG TTA TC - 3'

Reverse Primer

5'-GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TGT TAA ATA TAG ATC ACA ATT TTG AAA CCG- 3'

Source

This part was PCRed out of E. coli's genome with the primers listed in the design notes section.

References

<biblio>

  1. Yim-1994 pmid=8282684
  2. Schellhorn-1998 pmid=9829938
  3. Wise pmid=8631665
  4. Funabashi pmid=11879714
  5. Makinoshima pmid=11985708
  6. Yim-1992 pmid=1317380
  7. Hengge-Aronis-1993 pmid=8416901
  8. Vijaykumar-2004 pmid=15576800

</biblio>

[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=453110 S67278]

Neidhart, Frederick C. Escherichia coli and Salmonella. Washington: ASM Press, 1996.