Difference between revisions of "Part:BBa J45009"
 
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<partinfo>BBa_J45009 short</partinfo>
 
<partinfo>BBa_J45009 short</partinfo>
  
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*'''Very important note: None of the indicated base changes were made.  Please consult design page to determine how we worked with this part.'''
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<partinfo>BBa_J45009</partinfo> encodes a branched-chain amino acid transaminase derived from the ''THI3'' gene from ''Saccharomyces cerevisiae''.  THI3 catalyzes the second step in isoamyl alcohol biosynthesis: conversion of &alpha;-ketoisocaproate to 3-methylbutanal via enzymatic reaction 4.1.1.1.
  
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This part catalyzes the second step in isoamyl alcohol biosynthesis.  It converts alpha-ketoisocaproate to 3-methylbutanal via enzymatic reaction 4.1.1.1.  3-methylbutanal is then reduced via a alcohol dehydrogenase to isoamyl alcohol (the precursor to isoamyl acetate or banana scent).
 
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===Usage and Biology===
 
===Usage and Biology===
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*''THI3'' is formerly known as ''KID1''.
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*3-methylbutanal is reduced via a chassis alcohol dehydrogenase to isoamyl alcohol which is a precursor to isoamyl acetate (banana odor).
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*'''Note: <partinfo>BBa_J45009</partinfo> has several internal BioBrick restriction sites.  We indicate proposed silent mutations for eliminating the BioBrick restriction sites.  It can only be used as a suffix part in BioBrick assembly.'''
  
 
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Latest revision as of 22:43, 7 March 2008

ketoisocaproate decarboxylase (THI3); converts alpha-ketoisocaproate to 3-methylbutanal

BBa_J45009 encodes a branched-chain amino acid transaminase derived from the THI3 gene from Saccharomyces cerevisiae. THI3 catalyzes the second step in isoamyl alcohol biosynthesis: conversion of α-ketoisocaproate to 3-methylbutanal via enzymatic reaction 4.1.1.1.

Usage and Biology

  • THI3 is formerly known as KID1.
  • 3-methylbutanal is reduced via a chassis alcohol dehydrogenase to isoamyl alcohol which is a precursor to isoamyl acetate (banana odor).
  • Note: BBa_J45009 has several internal BioBrick restriction sites. We indicate proposed silent mutations for eliminating the BioBrick restriction sites. It can only be used as a suffix part in BioBrick assembly.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3
    Illegal EcoRI site found at 904
    Illegal EcoRI site found at 1603
    Illegal EcoRI site found at 1789
    Illegal SpeI site found at 697
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3
    Illegal EcoRI site found at 904
    Illegal EcoRI site found at 1603
    Illegal EcoRI site found at 1789
    Illegal SpeI site found at 697
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3
    Illegal EcoRI site found at 904
    Illegal EcoRI site found at 1603
    Illegal EcoRI site found at 1789
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3
    Illegal EcoRI site found at 904
    Illegal EcoRI site found at 1603
    Illegal EcoRI site found at 1789
    Illegal SpeI site found at 697
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3
    Illegal EcoRI site found at 904
    Illegal EcoRI site found at 1603
    Illegal EcoRI site found at 1789
    Illegal SpeI site found at 697
  • 1000
    COMPATIBLE WITH RFC[1000]