Difference between revisions of "Part:BBa J45004:Design"
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====Template==== | ====Template==== | ||
| − | + | pET-28a-''BSMT1''<cite>Negre03</cite> | |
| − | + | *We thank Natalia Dudareva, Department of Horticulture and Landscape Architecture, Purdue University for the gift of the expression vector with the petunia ''BSMT1'' coding sequence. | |
====Forward primer==== | ====Forward primer==== | ||
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===Source=== | ===Source=== | ||
| − | *''BSMT1'' from ''Petunia x hybrida'' (GenBank [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&val=28629494 AY233465]) | + | *''BSMT1'' gene from ''Petunia x hybrida'' (GenBank [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&val=28629494 AY233465]) |
===References=== | ===References=== | ||
Latest revision as of 22:29, 7 March 2008
SAM:benzoic acid/salicylic acid carboxyl methyltransferase I; converts salicylic acid to methyl sali
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 663
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 204
Design Notes
Constructed by PCR.
Template
pET-28a-BSMT1Negre03
- We thank Natalia Dudareva, Department of Horticulture and Landscape Architecture, Purdue University for the gift of the expression vector with the petunia BSMT1 coding sequence.
Forward primer
5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAT GGA AGT TGT TGA AGT TC -3'
Reverse primer
5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAA TTT ATT TTG GTC AAG GAG -3'
Source
- BSMT1 gene from Petunia x hybrida (GenBank [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&val=28629494 AY233465])
References
<biblio>
- Underwood05 pmid=15849311
- Negre03 pmid=14630969
</biblio>