Difference between revisions of "Part:BBa J45004:Design"
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===Design Notes===
 
===Design Notes===
 +
Constructed by PCR.
 +
 +
====Template====
 +
pET-28a-''BSMT1''<cite>Negre03</cite>
 +
*We thank Natalia Dudareva, Department of Horticulture and Landscape Architecture, Purdue University for the gift of the expression vector with the petunia ''BSMT1'' coding sequence.
  
 
====Forward primer====  
 
====Forward primer====  
−
<code>5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA G '''ATG GAA GTT GTT GAA GTT C''' -3'</code>
+
<code>5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA G'''AT GGA AGT TGT TGA AGT TC''' -3'</code>
−
*LENGTH: 19
+
−
*GC CONTENT: 36.8%
+
−
*MELT TEMP: 47.8&deg;C
+
  
 
====Reverse primer====
 
====Reverse primer====
 
<code>5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TA'''T TAA TTT ATT TTG GTC AAG GAG''' -3'</code>
 
<code>5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TA'''T TAA TTT ATT TTG GTC AAG GAG''' -3'</code>
−
*LENGTH: 22
 
−
*GC CONTENT: 27.3%
 
−
*MELT TEMP: 46.6&deg;C
 
  
 
===Source===
 
===Source===
−
 
+
*''BSMT1'' gene from ''Petunia x hybrida'' (GenBank [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&val=28629494 AY233465])
−
Species: ''Petunia x hybrida'' from GenBank ([http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&val=28629494 AY233465]); (also available: ''A. thaliana'' and ''N. suaveolens'')
+
  
 
===References===
 
===References===

Latest revision as of 22:29, 7 March 2008

SAM:benzoic acid/salicylic acid carboxyl methyltransferase I; converts salicylic acid to methyl sali


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 663
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 204


Design Notes

Constructed by PCR.

Template

pET-28a-BSMT1Negre03

  • We thank Natalia Dudareva, Department of Horticulture and Landscape Architecture, Purdue University for the gift of the expression vector with the petunia BSMT1 coding sequence.

Forward primer

5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAT GGA AGT TGT TGA AGT TC -3'

Reverse primer

5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAA TTT ATT TTG GTC AAG GAG -3'

Source

  • BSMT1 gene from Petunia x hybrida (GenBank [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&val=28629494 AY233465])

References

<biblio>

  1. Underwood05 pmid=15849311
  2. Negre03 pmid=14630969

</biblio>