Difference between revisions of "Part:BBa J45004:Design"

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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_J45004 short</partinfo>
 
<partinfo>BBa_J45004 short</partinfo>
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===Design Notes===
 
===Design Notes===
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Just CDS taken from GenBank
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Constructed by PCR.
  
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Designed Forward Strand (1 and 2):
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====Template====
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5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG <b>ATG GAA GTT GTT GAA GTT C</b> -3'<br>
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pET-28a-''BSMT1''<cite>Negre03</cite>
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*LENGTH: 49<br>
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*We thank Natalia Dudareva, Department of Horticulture and Landscape Architecture, Purdue University for the gift of the expression vector with the petunia ''BSMT1'' coding sequence.
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*GC CONTENT: 46.9 %<br>
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*MELT TEMP: 67.6 ºC<br>
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<br>
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Designed Reverse Strand (phBSMT1): 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TA<b>T TAT TAA TTT ATT TTG GTC AAC</b> -3'<br>
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*LENGTH: 51<br>
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*GC CONTENT: 39.2 %<br>
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*MELT TEMP:<br>
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===Source===
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====Forward primer====
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<code>5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA G'''AT GGA AGT TGT TGA AGT TC''' -3'</code>
  
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Species: Petunia x hybrida from GenBank (AY233465); (also available: A. thaliana and N. suaveolens)
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====Reverse primer====
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<code>5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TA'''T TAA TTT ATT TTG GTC AAG GAG''' -3'</code>
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===Source===
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*''BSMT1'' gene from ''Petunia x hybrida'' (GenBank [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&val=28629494 AY233465])
  
 
===References===
 
===References===
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#*<biblio>Underwood05 pmid=15849311</biblio>
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<biblio>
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#*<biblio>Negre03 pmid=14630969</biblio>
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#Underwood05 pmid=15849311
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#Negre03 pmid=14630969
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</biblio>

Latest revision as of 22:29, 7 March 2008

SAM:benzoic acid/salicylic acid carboxyl methyltransferase I; converts salicylic acid to methyl sali


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 663
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 204


Design Notes

Constructed by PCR.

Template

pET-28a-BSMT1Negre03

  • We thank Natalia Dudareva, Department of Horticulture and Landscape Architecture, Purdue University for the gift of the expression vector with the petunia BSMT1 coding sequence.

Forward primer

5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAT GGA AGT TGT TGA AGT TC -3'

Reverse primer

5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAA TTT ATT TTG GTC AAG GAG -3'

Source

  • BSMT1 gene from Petunia x hybrida (GenBank [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&val=28629494 AY233465])

References

<biblio>

  1. Underwood05 pmid=15849311
  2. Negre03 pmid=14630969

</biblio>