Difference between revisions of "Part:BBa J45014:Design"
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===Source=== | ===Source=== | ||
| − | ''ATF1'' gene | + | ''ATF1'' gene from ''Saccharomyces cerevisiae'' (GenBank [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=Nucleotide&dopt=GenBank&val=1420813 Z75285]) |
===References=== | ===References=== | ||
<biblio> | <biblio> | ||
| + | #Fujii--1984 pmid=8085822 | ||
#Horton03 pmid=12937998 | #Horton03 pmid=12937998 | ||
#Singh--2007 pmid=17891501 | #Singh--2007 pmid=17891501 | ||
</biblio> | </biblio> | ||
Latest revision as of 22:10, 7 March 2008
alcohol acetyltransferase I; converts isoamyl alcohol to isoamyl acetate (banana odor)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 99
Illegal BamHI site found at 1426 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 526
Illegal SapI.rc site found at 1534
Design Notes
- Constructed by PCR.
- Eliminated EcoRI site at base pair 412 by changing base pair 414 from A to G
- BBa_J45014 differs from BBa_J45006 by a single point mutation at base pair 1148 (T -> C).
Template
Saccharomyces cerevisiae strain ACLY387 genome
Forward primer
5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAT GAA TGA AAT CGA TGA GAA AAA TC -3'
Reverse primer
5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAa ggg cct aaa agg aga gct ttg -3'
Forward mutagenesis primer
5'- GAA GCA AAT ATT AGA AGA GTT CAA AAA TAG TAA GGG -3'
Reverse mutagenesis primer
5'- CCC TTA CTA TTT TTG AAC TCT TCT AAT ATT TGC TTC -3'
Source
ATF1 gene from Saccharomyces cerevisiae (GenBank [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=Nucleotide&dopt=GenBank&val=1420813 Z75285])
References
<biblio>
- Fujii--1984 pmid=8085822
- Horton03 pmid=12937998
- Singh--2007 pmid=17891501
</biblio>