Difference between revisions of "Part:BBa J45004:Design"

(→‎Forward primer)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
 +
 +
====Template====
 +
pET-28a-''BSMT1''
  
 
====Forward primer====  
 
====Forward primer====  
 
<code>5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA G'''AT GGA AGT TGT TGA AGT TC''' -3'</code>
 
<code>5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA G'''AT GGA AGT TGT TGA AGT TC''' -3'</code>
−
*LENGTH: 19
 
−
*GC CONTENT: 36.8%
 
−
*MELT TEMP: 47.8&deg;C
 
  
 
====Reverse primer====
 
====Reverse primer====
 
<code>5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TA'''T TAA TTT ATT TTG GTC AAG GAG''' -3'</code>
 
<code>5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TA'''T TAA TTT ATT TTG GTC AAG GAG''' -3'</code>
−
*LENGTH: 22
 
−
*GC CONTENT: 27.3%
 
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*MELT TEMP: 46.6&deg;C
 
  
 
===Source===
 
===Source===

Revision as of 21:55, 7 March 2008

SAM:benzoic acid/salicylic acid carboxyl methyltransferase I; converts salicylic acid to methyl sali


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 663
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 204


Design Notes

Template

pET-28a-BSMT1

Forward primer

5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAT GGA AGT TGT TGA AGT TC -3'

Reverse primer

5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAA TTT ATT TTG GTC AAG GAG -3'

Source

  • BSMT1 from Petunia x hybrida (GenBank [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&val=28629494 AY233465])

References

<biblio>

  1. Underwood05 pmid=15849311
  2. Negre03 pmid=14630969

</biblio>