Difference between revisions of "Part:BBa J45004:Design"
(→‎Forward primer)
Line 8: Line 8:
  
 
====Forward primer====  
 
====Forward primer====  
−
<code>5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA G '''ATG GAA GTT GTT GAA GTT C''' -3'</code>
+
<code>5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA G'''AT GGA AGT TGT TGA AGT TC''' -3'</code>
 
*LENGTH: 19
 
*LENGTH: 19
 
*GC CONTENT: 36.8%
 
*GC CONTENT: 36.8%

Revision as of 21:53, 7 March 2008

SAM:benzoic acid/salicylic acid carboxyl methyltransferase I; converts salicylic acid to methyl sali


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 663
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 204


Design Notes

Forward primer

5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAT GGA AGT TGT TGA AGT TC -3'

  • LENGTH: 19
  • GC CONTENT: 36.8%
  • MELT TEMP: 47.8°C

Reverse primer

5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAA TTT ATT TTG GTC AAG GAG -3'

  • LENGTH: 22
  • GC CONTENT: 27.3%
  • MELT TEMP: 46.6°C

Source

  • BSMT1 from Petunia x hybrida (GenBank [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&val=28629494 AY233465])

References

<biblio>

  1. Underwood05 pmid=15849311
  2. Negre03 pmid=14630969

</biblio>