Difference between revisions of "Part:BBa J45004:Design"
| Line 8: | Line 8: | ||
====Forward primer==== | ====Forward primer==== | ||
| − | <code>5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA G '''ATG GAA GTT GTT GAA GTT C''' -3'<code> | + | <code>5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA G '''ATG GAA GTT GTT GAA GTT C''' -3'</code> |
*LENGTH: 19 | *LENGTH: 19 | ||
*GC CONTENT: 36.8% | *GC CONTENT: 36.8% | ||
| Line 18: | Line 18: | ||
*GC CONTENT: 27.3% | *GC CONTENT: 27.3% | ||
*MELT TEMP: 46.6°C | *MELT TEMP: 46.6°C | ||
| − | |||
===Source=== | ===Source=== | ||
Revision as of 21:47, 7 March 2008
SAM:benzoic acid/salicylic acid carboxyl methyltransferase I; converts salicylic acid to methyl sali
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 663
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 204
Design Notes
Forward primer
5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA G ATG GAA GTT GTT GAA GTT C -3'
- LENGTH: 19
- GC CONTENT: 36.8%
- MELT TEMP: 47.8°C
Reverse primer
5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAA TTT ATT TTG GTC AAG GAG -3'
- LENGTH: 22
- GC CONTENT: 27.3%
- MELT TEMP: 46.6°C
Source
Species: Petunia x hybrida from GenBank ([http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&val=28629494 AY233465]); (also available: A. thaliana and N. suaveolens)
References
<biblio>
- Underwood05 pmid=15849311
- Negre03 pmid=14630969
</biblio>