Difference between revisions of "Part:BBa J45004:Design"

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===Design Notes===
 
===Design Notes===
−
Just CDS taken from GenBank
 
  
−
Designed Forward Strand (1 and 2):
+
====Forward primer====
−
5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA G <b>ATG GAA GTT GTT GAA GTT C</b> -3'<br>
+
<code>5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA G '''ATG GAA GTT GTT GAA GTT C''' -3'<code>
−
*LENGTH: 19 <br>
+
*LENGTH: 19
−
*GC CONTENT: 36.8 % <br>
+
*GC CONTENT: 36.8%
−
*MELT TEMP: 47.8 ºC <br>
+
*MELT TEMP: 47.8&deg;C
−
<br>
+
 
−
Designed Reverse Strand (phBSMT1): 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TA<b>T TAA TTT ATT TTG GTC AAG GAG</b> -3'<br>
+
====Reverse primer====
−
*LENGTH: 22 <Br>
+
<code>5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TA'''T TAA TTT ATT TTG GTC AAG GAG''' -3'</code>
−
*GC CONTENT: 27.3 % <br>
+
*LENGTH: 22
−
*MELT TEMP: 46.6 ºC <br>
+
*GC CONTENT: 27.3%
 +
*MELT TEMP: 46.6&deg;C
 
<br>
 
<br>
  

Revision as of 21:46, 7 March 2008

SAM:benzoic acid/salicylic acid carboxyl methyltransferase I; converts salicylic acid to methyl sali


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 663
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 204


Design Notes

Forward primer

5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA G ATG GAA GTT GTT GAA GTT C -3'<code>

  • LENGTH: 19
  • GC CONTENT: 36.8%
  • MELT TEMP: 47.8°C

Reverse primer

<code>5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAA TTT ATT TTG GTC AAG GAG -3'

  • LENGTH: 22
  • GC CONTENT: 27.3%
  • MELT TEMP: 46.6°C


Source

Species: Petunia x hybrida from GenBank ([http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&val=28629494 AY233465]); (also available: A. thaliana and N. suaveolens)

References

<biblio>

  1. Underwood05 pmid=15849311
  2. Negre03 pmid=14630969

</biblio>