Difference between revisions of "Part:BBa K2842666"
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<p>
 
<p>
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(1) BsaI digested Ba_I732902 (Golden Gate) <br />
+
(1) BsaI digested [https://parts.igem.org/Part:Ba_I732902 Ba_I732902] (Golden Gate) <br />
 
(2) BsaI digested Final Brick (Golden Gate) <br />
 
(2) BsaI digested Final Brick (Golden Gate) <br />
−
(3) EcoRI+PstI digested Ba_I732902 (BioBrick Assembly) <br />
+
(3) EcoRI+PstI digested [https://parts.igem.org/Part:Ba_I732902 Ba_I732902] (BioBrick Assembly) <br />
 
(4) EcoRI+PstI digested Final Brick (BioBrick Assembly) <br />
 
(4) EcoRI+PstI digested Final Brick (BioBrick Assembly) <br />
−
(5) undigested Ba_I732902 <br />
+
(5) undigested [https://parts.igem.org/Part:Ba_I732902 Ba_I732902] <br />
 
(6) undigested Final Brick
 
(6) undigested Final Brick
 
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</p>''' ]]

Revision as of 15:20, 5 October 2018


Golden Gate compatible system with blue-white screening

Flexible Cloning System
Function Standardised blue/white measurement
Use in E. coli cells
Chassis Tested DH5α cells
Abstraction Hierarchy Composite Part
Related Device BBa_I732902
RFC standard RFC10,RFC12,RFC21,RFC23 & RFC25 compatible
Backbone pSB1C3
Submitted by [http://2018.igem.org/Team:UCL UCL iGEM 2018]


Figure 1: Final Brick and Ba_I732902 cloning comparison

(1) BsaI digested Ba_I732902 (Golden Gate)
(2) BsaI digested Final Brick (Golden Gate)
(3) EcoRI+PstI digested Ba_I732902 (BioBrick Assembly)
(4) EcoRI+PstI digested Final Brick (BioBrick Assembly)
(5) undigested Ba_I732902
(6) undigested Final Brick





























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Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 647
    Illegal BsaI.rc site found at 28