Difference between revisions of "Part:BBa K2842666"

Revision as of 12:56, 5 October 2018

Golden Gate compatible system with blue-white screening

Flexible Cloning System
Function	Standardised blue/white measurement
Use in	E. coli cells
Chassis Tested	DH5α cells
Abstraction Hierarchy	Composite Part
Related Device	BBa_I732902
RFC standard	RFC10,RFC12,RFC21,RFC23 & RFC25 compatible
Backbone	pSB1C3
Submitted by	[http://2018.igem.org/Team:UCL UCL iGEM 2018]

Figure 1: Final Brick and Ba_I732902 cloning comparison

(1) BsaI digested Ba_I732902 (Golden Gate) (2) BsaI digested Final Brick (Golden Gate) (3) EcoRI+PstI digested Ba_I732902 (BioBrick Assembly) (4) EcoRI+PstI digested Final Brick (BioBrick Assembly) (5) undigested Ba_I732902 (6) undigested Final Brick

-

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 647
Illegal BsaI.rc site found at 28

@@ Line 32: / Line 32: @@
 [[File:T--UCL--FB gelfig.png|400px|thumb|left|
-<center>'''Figure 1: Final Brick and lacZ cloning comparison'''</center>
+<center>'''Figure 1: Final Brick and Ba_I732902 cloning comparison'''</center>
 <p>
-(1) ...(2)...
+(1) BsaI digested Ba_I732902 (Golden Gate)
+(2) BsaI digested Final Brick (Golden Gate)
+(3) EcoRI+PstI digested Ba_I732902 (BioBrick Assembly)
+(4) EcoRI+PstI digested Final Brick (BioBrick Assembly)
+(5) undigested Ba_I732902
+(6) undigested Final Brick
 </p>''' ]]